Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans

Palika Wetchakul, Goon Jo Aan, Ademola Ezekiel Adekoya, Opeyemi Joshua Olatunji, Sutticha Ruangchuay, Patcharawalai Jaisamut, Acharaporn Issuriya, Nongluk Kunworarath, Surasak Limsuwan, Sasitorn Chusri

Research output: Contribution to journalArticle

Abstract

Background: The imbalance between the generation of free radicals and natural cellular antioxidant defenses, known as oxidative stress, can cause oxidation of biomolecules and further contribute to aging-associated diseases. The purpose of this study was to evaluate the antioxidant capacities of Thai traditional tonifying preparation, Jatu-Phala-Tiga (JPT) and its herbal ingredients consisting of Phyllanthus emblica, Terminalia arjuna, Terminalia chebula, and Terminalia bellirica and further assess its effect on longevity. Method: Antioxidant activities of various extracts obtained from JPT and its herbal components were carried out using well-established methods including metal chelating, free radical scavenging, and ferric reducing antioxidant power assays. Qualitative analysis of the chemical composition from JPT water extract was done by high-performance liquid chromatography tandem with electrospray ionisation mass spectrometry. The effect of JPT water extract on the lifespan of Caenorhabditis elegans were additionally described. Results: Among the extracts, JPT water extract exerted remarkable antioxidant activities as compared to the extracts from other solvents and individual constituting plant extract. JPT water extract was found to possess the highest metal chelating activity, with an IC50 value of 1.75 ± 0.05 mg/mL. Moreover, it exhibited remarkable scavenging activities towards DPPH, ABTS, and superoxide anion radicals, with IC50 values of 0.31 ± 0.02, 0.308 ± 0.004, and 0.055 ± 0.002 mg/mL, respectively. The ORAC and FRAP values of JPT water extract were 40.338 ± 2.273 μM of Trolox/μg of extract and 23.07 ± 1.84 mM FeSO4/mg sample, respectively. Several well-known antioxidant-related compounds including amaronols, quinic acid, gallic acid, fertaric acid, kurigalin, amlaic acid, isoterchebin, chebulagic acid, ginkgolide C, chebulinic acid, ellagic acid, and rutin were found in this extract. Treatment with JPT water extract at 1 and 5 mg/mL increased C. elegans lifespan under normal growth condition (7.26 ± 0.65 vs. 10.4 0± 0.75 (p < 0.01) and 10.00 ± 0.73 (p < 0.01) days, respectively). Conclusions: The results indicated that JPT and its herbal ingredients exhibited strong antioxidant activities, in particular the water extract of the polyherbal tonic. These findings rationalize further investigation in JPT infusion as a promising agent for anti-aging and oxidative stress prevention.

Original languageEnglish
Article number209
JournalBMC Complementary and Alternative Medicine
Volume19
Issue number1
DOIs
Publication statusPublished - 13 Aug 2019

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Caenorhabditis elegans
Antioxidants
Terminalia
Water
Superoxides
Inhibitory Concentration 50
Free Radicals
Oxidative Stress
Phyllanthus emblica
Metals
Quinic Acid
Ellagic Acid
Rutin
Gallic Acid
Electrospray Ionization Mass Spectrometry
Plant Extracts
High Pressure Liquid Chromatography
Acids
Growth

Keywords

  • Antioxidants
  • Caenorhabditis elegans
  • Herbal tonic
  • Polyherbal formula
  • Tonifying agents

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans. / Wetchakul, Palika; Jo Aan, Goon; Adekoya, Ademola Ezekiel; Olatunji, Opeyemi Joshua; Ruangchuay, Sutticha; Jaisamut, Patcharawalai; Issuriya, Acharaporn; Kunworarath, Nongluk; Limsuwan, Surasak; Chusri, Sasitorn.

In: BMC Complementary and Alternative Medicine, Vol. 19, No. 1, 209, 13.08.2019.

Research output: Contribution to journalArticle

Wetchakul, P, Jo Aan, G, Adekoya, AE, Olatunji, OJ, Ruangchuay, S, Jaisamut, P, Issuriya, A, Kunworarath, N, Limsuwan, S & Chusri, S 2019, 'Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans', BMC Complementary and Alternative Medicine, vol. 19, no. 1, 209. https://doi.org/10.1186/s12906-019-2626-1
Wetchakul, Palika ; Jo Aan, Goon ; Adekoya, Ademola Ezekiel ; Olatunji, Opeyemi Joshua ; Ruangchuay, Sutticha ; Jaisamut, Patcharawalai ; Issuriya, Acharaporn ; Kunworarath, Nongluk ; Limsuwan, Surasak ; Chusri, Sasitorn. / Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans. In: BMC Complementary and Alternative Medicine. 2019 ; Vol. 19, No. 1.
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T1 - Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans

AU - Wetchakul, Palika

AU - Jo Aan, Goon

AU - Adekoya, Ademola Ezekiel

AU - Olatunji, Opeyemi Joshua

AU - Ruangchuay, Sutticha

AU - Jaisamut, Patcharawalai

AU - Issuriya, Acharaporn

AU - Kunworarath, Nongluk

AU - Limsuwan, Surasak

AU - Chusri, Sasitorn

PY - 2019/8/13

Y1 - 2019/8/13

N2 - Background: The imbalance between the generation of free radicals and natural cellular antioxidant defenses, known as oxidative stress, can cause oxidation of biomolecules and further contribute to aging-associated diseases. The purpose of this study was to evaluate the antioxidant capacities of Thai traditional tonifying preparation, Jatu-Phala-Tiga (JPT) and its herbal ingredients consisting of Phyllanthus emblica, Terminalia arjuna, Terminalia chebula, and Terminalia bellirica and further assess its effect on longevity. Method: Antioxidant activities of various extracts obtained from JPT and its herbal components were carried out using well-established methods including metal chelating, free radical scavenging, and ferric reducing antioxidant power assays. Qualitative analysis of the chemical composition from JPT water extract was done by high-performance liquid chromatography tandem with electrospray ionisation mass spectrometry. The effect of JPT water extract on the lifespan of Caenorhabditis elegans were additionally described. Results: Among the extracts, JPT water extract exerted remarkable antioxidant activities as compared to the extracts from other solvents and individual constituting plant extract. JPT water extract was found to possess the highest metal chelating activity, with an IC50 value of 1.75 ± 0.05 mg/mL. Moreover, it exhibited remarkable scavenging activities towards DPPH, ABTS, and superoxide anion radicals, with IC50 values of 0.31 ± 0.02, 0.308 ± 0.004, and 0.055 ± 0.002 mg/mL, respectively. The ORAC and FRAP values of JPT water extract were 40.338 ± 2.273 μM of Trolox/μg of extract and 23.07 ± 1.84 mM FeSO4/mg sample, respectively. Several well-known antioxidant-related compounds including amaronols, quinic acid, gallic acid, fertaric acid, kurigalin, amlaic acid, isoterchebin, chebulagic acid, ginkgolide C, chebulinic acid, ellagic acid, and rutin were found in this extract. Treatment with JPT water extract at 1 and 5 mg/mL increased C. elegans lifespan under normal growth condition (7.26 ± 0.65 vs. 10.4 0± 0.75 (p < 0.01) and 10.00 ± 0.73 (p < 0.01) days, respectively). Conclusions: The results indicated that JPT and its herbal ingredients exhibited strong antioxidant activities, in particular the water extract of the polyherbal tonic. These findings rationalize further investigation in JPT infusion as a promising agent for anti-aging and oxidative stress prevention.

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KW - Antioxidants

KW - Caenorhabditis elegans

KW - Herbal tonic

KW - Polyherbal formula

KW - Tonifying agents

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