Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential

Geita Saadatnia, Zeehaida Mohamed, Fatemeh Ghaffarifar, Emelia Osman, Zohreh Kazemi Moghadam, Rahmah Noordin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Infection with Toxoplasma gondii is widespread and important in humans, especially pregnant women and immunosuppressed patients. A panel of tests is usually required for diagnosis toxoplasmosis. Excretory secretory antigen (ESA) is highly immunogenic, and thus it is a good candidate for investigation into new infection markers. ESA was prepared from tachyzoites of RH strain of T. gondii by mice intraperitoneal infection. Sera were obtained from several categories of individuals who differed in their status of anti-Toxoplasma IgM, IgG and IgG avidity antibodies. The ESA was subjected to SDS-PAGE, two-dimensional gel electrophoresis and Western blot analysis. Antigenic bands of approximate molecular weights of 12, 20 and 30kDa, when probed with anti-human IgM-HRP and IgA-HRP, showed good potential as infection markers. The highest sensitivity of the bands was 98.7% with combination of IgM and IgA blots with sera of patients with anti-Toxoplasma IgM+ IgG+. The specificities were 84% and 70% with sera from other infections and healthy controls in IgM blots and IgA blots respectively. By mass spectrometry, the 12kDa protein was identified as thioredoxin. The two top proteins identified for 20kDa molecule were microneme protein 10 and dense granule protein 7; whereas that for 30kDa were phosphoglycerate mutase 1 and phosphoglycerate mutase.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalAPMIS
Volume120
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Fingerprint

Toxoplasma
Phosphoglycerate Mutase
Immunoglobulin A
Toxoplasmosis
Antigens
Immunoglobulin M
Proteins
Immunoglobulin G
Infection
Serum
Thioredoxins
Antibody Affinity
Electrophoresis, Gel, Two-Dimensional
Infection Control
Pregnant Women
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Molecular Weight
Western Blotting
anti-IgM

Keywords

  • 2-DE
  • Diagnosis
  • Excretory secretory antigen
  • Mass spectrometry
  • Toxoplasma gondii
  • Western blot

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)
  • Immunology and Allergy

Cite this

Saadatnia, G., Mohamed, Z., Ghaffarifar, F., Osman, E., Moghadam, Z. K., & Noordin, R. (2012). Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential. APMIS, 120(1), 47-55. https://doi.org/10.1111/j.1600-0463.2011.02810.x

Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential. / Saadatnia, Geita; Mohamed, Zeehaida; Ghaffarifar, Fatemeh; Osman, Emelia; Moghadam, Zohreh Kazemi; Noordin, Rahmah.

In: APMIS, Vol. 120, No. 1, 01.2012, p. 47-55.

Research output: Contribution to journalArticle

Saadatnia, G, Mohamed, Z, Ghaffarifar, F, Osman, E, Moghadam, ZK & Noordin, R 2012, 'Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential', APMIS, vol. 120, no. 1, pp. 47-55. https://doi.org/10.1111/j.1600-0463.2011.02810.x
Saadatnia, Geita ; Mohamed, Zeehaida ; Ghaffarifar, Fatemeh ; Osman, Emelia ; Moghadam, Zohreh Kazemi ; Noordin, Rahmah. / Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential. In: APMIS. 2012 ; Vol. 120, No. 1. pp. 47-55.
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