Toxicity mechanism of triphenyltin (IV) butylphenyldithiocarbamate on acute lymphoblastic cells, Jurkat E6.1

Research output: Contribution to journalArticle

Abstract

Organotin(IV) derivatives have emerged as potential metallopharmaceuticals due to their efficacy to induce cytotoxicity in various types of cancerous cell lines. Triphenyltin(IV) butylphenyldithiocarbamate (TFBF), a novel compound has been found to exhibit primary apoptosis in Jurkat E6.1 cells with IC50 of 0.4 μM. In this study, the role of reactive oxygen species (ROS) and loss of mitochondrial membrane potential (Δψm) in Jurkat E6.1 cells were assessed along with activation of caspase-3. The measurement of ROS and the loss of ΔΨm were conducted using dihydroethidium (HE) staining assay and tetramethylrhodamine ethyl ester (TMRE) staining assay, respectively. The cells were treated in the time series from 1/2 h up to 4 h prior to flow cytometric quantification. Caspase-3 activation from 1 h up to 6 h was evaluated using Caspase-Glo® Luminescence Kit. The results showed that there was an early increase of positive-HE stained cells as early as 1 h of treatment as compared to the negative control. Increased level of ROS led to the loss of Δψm. Activated caspase-3 was significant at 4 h of treatment. In conclusion, TFBF-induced apoptosis in Jurkat E6.1 cells via early production of ROS with loss of Δψm finally leads to caspase-3 activation.

Original languageEnglish
Pages (from-to)1457-1465
Number of pages9
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Volume6
Issue number2
Publication statusPublished - 2015

Fingerprint

Jurkat Cells
Caspase 3
Toxicity
Reactive Oxygen Species
Chemical activation
Assays
Apoptosis
Staining and Labeling
Mitochondrial Membrane Potential
Cytotoxicity
Caspases
Luminescence
Inhibitory Concentration 50
Time series
Esters
Cells
Derivatives
Membranes
Cell Line
triphenyltin

Keywords

  • Apoptosis
  • Cytotoxicity
  • Dithiocarbamate
  • Jurkat E6.1
  • Organotin(IV)

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{bdd7137b5d9549a3a4d43439aeb61d7b,
title = "Toxicity mechanism of triphenyltin (IV) butylphenyldithiocarbamate on acute lymphoblastic cells, Jurkat E6.1",
abstract = "Organotin(IV) derivatives have emerged as potential metallopharmaceuticals due to their efficacy to induce cytotoxicity in various types of cancerous cell lines. Triphenyltin(IV) butylphenyldithiocarbamate (TFBF), a novel compound has been found to exhibit primary apoptosis in Jurkat E6.1 cells with IC50 of 0.4 μM. In this study, the role of reactive oxygen species (ROS) and loss of mitochondrial membrane potential (Δψm) in Jurkat E6.1 cells were assessed along with activation of caspase-3. The measurement of ROS and the loss of ΔΨm were conducted using dihydroethidium (HE) staining assay and tetramethylrhodamine ethyl ester (TMRE) staining assay, respectively. The cells were treated in the time series from 1/2 h up to 4 h prior to flow cytometric quantification. Caspase-3 activation from 1 h up to 6 h was evaluated using Caspase-Glo{\circledR} Luminescence Kit. The results showed that there was an early increase of positive-HE stained cells as early as 1 h of treatment as compared to the negative control. Increased level of ROS led to the loss of Δψm. Activated caspase-3 was significant at 4 h of treatment. In conclusion, TFBF-induced apoptosis in Jurkat E6.1 cells via early production of ROS with loss of Δψm finally leads to caspase-3 activation.",
keywords = "Apoptosis, Cytotoxicity, Dithiocarbamate, Jurkat E6.1, Organotin(IV)",
author = "Normah Awang and {Abd Kadir}, {Nur Shuhada} and {Nurul Farahana}, Kamaludin and {Kok Meng}, Chan",
year = "2015",
language = "English",
volume = "6",
pages = "1457--1465",
journal = "Research Journal of Pharmaceutical, Biological and Chemical Sciences",
issn = "0975-8585",
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}

TY - JOUR

T1 - Toxicity mechanism of triphenyltin (IV) butylphenyldithiocarbamate on acute lymphoblastic cells, Jurkat E6.1

AU - Awang, Normah

AU - Abd Kadir, Nur Shuhada

AU - Nurul Farahana, Kamaludin

AU - Kok Meng, Chan

PY - 2015

Y1 - 2015

N2 - Organotin(IV) derivatives have emerged as potential metallopharmaceuticals due to their efficacy to induce cytotoxicity in various types of cancerous cell lines. Triphenyltin(IV) butylphenyldithiocarbamate (TFBF), a novel compound has been found to exhibit primary apoptosis in Jurkat E6.1 cells with IC50 of 0.4 μM. In this study, the role of reactive oxygen species (ROS) and loss of mitochondrial membrane potential (Δψm) in Jurkat E6.1 cells were assessed along with activation of caspase-3. The measurement of ROS and the loss of ΔΨm were conducted using dihydroethidium (HE) staining assay and tetramethylrhodamine ethyl ester (TMRE) staining assay, respectively. The cells were treated in the time series from 1/2 h up to 4 h prior to flow cytometric quantification. Caspase-3 activation from 1 h up to 6 h was evaluated using Caspase-Glo® Luminescence Kit. The results showed that there was an early increase of positive-HE stained cells as early as 1 h of treatment as compared to the negative control. Increased level of ROS led to the loss of Δψm. Activated caspase-3 was significant at 4 h of treatment. In conclusion, TFBF-induced apoptosis in Jurkat E6.1 cells via early production of ROS with loss of Δψm finally leads to caspase-3 activation.

AB - Organotin(IV) derivatives have emerged as potential metallopharmaceuticals due to their efficacy to induce cytotoxicity in various types of cancerous cell lines. Triphenyltin(IV) butylphenyldithiocarbamate (TFBF), a novel compound has been found to exhibit primary apoptosis in Jurkat E6.1 cells with IC50 of 0.4 μM. In this study, the role of reactive oxygen species (ROS) and loss of mitochondrial membrane potential (Δψm) in Jurkat E6.1 cells were assessed along with activation of caspase-3. The measurement of ROS and the loss of ΔΨm were conducted using dihydroethidium (HE) staining assay and tetramethylrhodamine ethyl ester (TMRE) staining assay, respectively. The cells were treated in the time series from 1/2 h up to 4 h prior to flow cytometric quantification. Caspase-3 activation from 1 h up to 6 h was evaluated using Caspase-Glo® Luminescence Kit. The results showed that there was an early increase of positive-HE stained cells as early as 1 h of treatment as compared to the negative control. Increased level of ROS led to the loss of Δψm. Activated caspase-3 was significant at 4 h of treatment. In conclusion, TFBF-induced apoptosis in Jurkat E6.1 cells via early production of ROS with loss of Δψm finally leads to caspase-3 activation.

KW - Apoptosis

KW - Cytotoxicity

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