Toxicity biosensor for sodium dodecyl sulfate using immobilized green fluorescent protein expressing Escherichia coli

Lia Ooi, Yook Heng Lee, Asmat Ahmad

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Green fluorescent protein (GFP) is suitable as a toxicity sensor due to its ability to work alone without cofactors or substrates. Its reaction with toxicants can be determined with fluorometric approaches. GFP mutant gene (C48S/S147C/Q204C/S65T/Q80R) is used because it has higher sensitivity compared to others GFP variants. A novel sodium dodecyl sulfate (SDS) toxicity detection biosensor was built by immobilizing GFP expressing Escherichia coli in k-Carrageenan matrix. Cytotoxicity effect took place in the toxicity biosensor which leads to the decrease in the fluorescence intensity. The fabricated E. coli GFP toxicity biosensor has a wide dynamic range of 4-100 ppm, with LOD of 1.7 ppm. Besides, it possesses short response time (<1 min), high reproducibility (0.76% RSD) and repeatability (0.72% RSD, R2>0.98), and long-term stability (46 days). E. coli GFP toxicity biosensor has been applied to detect toxicity induced by SDS in tap water, river water, and drinking water. High recovery levels of SDS indicated the applicability of E. coli GFP toxicity biosensor in real water samples toxicity evaluation.

Original languageEnglish
Article number809065
JournalJournal of Sensors
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Sodium dodecyl sulfate
Escherichia
sodium sulfates
bioinstrumentation
Biosensors
toxicity
Escherichia coli
Toxicity
proteins
Proteins
water
Water
drinking
taps
Cytotoxicity
Potable water
genes
rivers
dynamic range
Genes

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

Toxicity biosensor for sodium dodecyl sulfate using immobilized green fluorescent protein expressing Escherichia coli. / Ooi, Lia; Lee, Yook Heng; Ahmad, Asmat.

In: Journal of Sensors, Vol. 2015, 809065, 2015.

Research output: Contribution to journalArticle

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