Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Taib Izatus Shima, Siti Balkis Budin, Seri Maseran Siti Nor Ain, Jamaludin Mohamed, Santhana Raj Louis, Srijit Das, Sulaiman Sallehudin, Nor Fadilah Rajab, Hidayatul Fathi Othman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

Original languageEnglish
Pages (from-to)813-819
Number of pages7
JournalJournal of Zhejiang University: Science B
Volume10
Issue number11
DOIs
Publication statusPublished - 2009

Fingerprint

Litsea
Poisons
Volatile Oils
Sprague Dawley Rats
Rats
essential oils
Blood
erythrocytes
Erythrocytes
Cells
hemoglobin
Erythrocyte Indices
rats
cell membranes
Hemoglobins
Osmotic Fragility
insecticidal properties
Control Groups
Erythrocyte Count
erythrocyte count

Keywords

  • Cell membrane
  • Echinocyte
  • Litsea elliptica
  • Morphology
  • Red blood cells
  • Toxicity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats. / Izatus Shima, Taib; Budin, Siti Balkis; Siti Nor Ain, Seri Maseran; Mohamed, Jamaludin; Louis, Santhana Raj; Das, Srijit; Sallehudin, Sulaiman; Rajab, Nor Fadilah; Othman, Hidayatul Fathi.

In: Journal of Zhejiang University: Science B, Vol. 10, No. 11, 2009, p. 813-819.

Research output: Contribution to journalArticle

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AU - Louis, Santhana Raj

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