Topical application of omega-3-, omega-6-, and omega-9-rich oil emulsions for cutaneous wound healing in rats

Wan Maznah Wan Ishak, Haliza Katas, Pei Yuen Ng, Maizaton Atmadini Abdullah, Mohd Hanif Zulfakar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Wound healing is a physiological event that generates reconstitution and restoration of granulation tissue that ends with scar formation. As omega fatty acids are part of membrane phospholipids and participate in the inflammatory response, we investigated the effects of omega-3, omega-6, and omega-9 fatty acids in the form of oils on wound healing. Linseed (LO), evening primrose (EPO), and olive oils (OO) rich in omega-3, omega-6, and omega-9 fatty acids were formulated into emulsions and were topically applied on rats with excision wounds. All omega-3-, omega-6-, and omega-9-rich oil formulations were found to accelerate wound closure compared to untreated, with significant improvement (p < 0.05) being observed at day 14. EPO induced early deposition of collagen as evaluated by Masson trichrome staining that correlated well with the hydroxyproline content assay, with the highest level at days 3 and 7. Vascular endothelial growth factor (VEGF) showed greater amount of new microvasculature formed in the EPO-treated group, while moderate improvement occurs in the LO and OO groups. EPO increased both the expression of proinflammatory cytokines and growth factors in the early stage of healing and declined at the later stage of healing. LO modulates the proinflammatory cytokines and chemokine but did not affect the growth factors. In contrast, OO induced the expression of growth factors rather than proinflammatory cytokines. These data suggest that LO, EPO, and OO emulsions promote wound healing but they accomplish this by different mechanisms.

Original languageEnglish
Pages (from-to)418-433
Number of pages16
JournalDrug Delivery and Translational Research
Volume9
Issue number2
DOIs
Publication statusPublished - 15 Apr 2019

Fingerprint

Oenothera biennis
Emulsions
Wound Healing
Oils
Omega-6 Fatty Acids
Intercellular Signaling Peptides and Proteins
Skin
Cytokines
Flax
Granulation Tissue
Hydroxyproline
Wounds and Injuries
Microvessels
Chemokines
Vascular Endothelial Growth Factor A
Cicatrix
Phospholipids
Collagen
Fatty Acids
Staining and Labeling

Keywords

  • Cytokines
  • Emulsion
  • Hydroxyproline
  • Omega oil
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Topical application of omega-3-, omega-6-, and omega-9-rich oil emulsions for cutaneous wound healing in rats. / Ishak, Wan Maznah Wan; Katas, Haliza; Ng, Pei Yuen; Abdullah, Maizaton Atmadini; Zulfakar, Mohd Hanif.

In: Drug Delivery and Translational Research, Vol. 9, No. 2, 15.04.2019, p. 418-433.

Research output: Contribution to journalArticle

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