Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture

E. A. Fellie, P. Sannasi, K. K. Wong, S. Salmijah, J. Kader

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed by toluene (IC50 = 139.67 mg/L), xylene (IC50 = 97.04 mg/L) and ethylbenzene (IC50 =96.99 mg/L). Significant decrease (p < 0.05) in the specific growth rate (μ), however was observed as the concentrations of each individual BTEX were increased from 10 mg/L to 500 mg/L. Growth of CC was completely inhibited at 250 mg/L ethylbenzene and 500 mg/L xylene. Toxicity followed the trend: B<T<X<E. Biodegradation of individual BTEX compound was monitored by gas chromatography. The GC-FID chromatographic profiles showed the capability of CC to significantly biodegrade (p < 0.05) benzene (61.66%), toluene (55.91%), ethylbenzene (37.15%), p-xylene (43.66%), m-xylene (47.86%) and o-xylene (41.03%) at an initial concentration of 50 mg/L after 48 hours. These findings confirm the ability of CC to withstand biodegrade and utilize BTEX as the sole source of carbon and energy in the following order: B>T>X>E.

    Original languageEnglish
    Pages (from-to)52-58
    Number of pages7
    JournalResearch Journal of Biotechnology
    Volume7
    Issue number3
    Publication statusPublished - Aug 2012

    Fingerprint

    Xylenes
    Ethylbenzene
    Toluene
    Xylene
    Biodegradation
    Benzene
    Inhibitory Concentration 50
    Metals
    Heavy Metals
    Isomers
    Heavy metals
    ethylbenzene

    Keywords

    • Bacteria
    • Biodegradation
    • Bioreme-diation
    • BTEX
    • BTEX tolerance

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture. / Fellie, E. A.; Sannasi, P.; Wong, K. K.; Salmijah, S.; Kader, J.

    In: Research Journal of Biotechnology, Vol. 7, No. 3, 08.2012, p. 52-58.

    Research output: Contribution to journalArticle

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