Tocotrienol-rich palm oil extract induces NAD(P)H: Quinone Oxidoreductase 1 (NQO1) expression in mice liver

Ahmed Atia, Nadia Alrawaiq, Azman Abdullah

Research output: Contribution to journalArticle

Abstract

A diet rich in tocotrienols has been shown to be beneficial for health. However, its detailed mechanism of action is still not fully understood. NAD(P)H:quinone oxidoreductase 1 (NQO1) is important in cellular defence due to its ability to detoxify reactive quinones and quinoneimines to their less toxic hydroquinones forms. The objective of this study is to investigate the effects of different doses of palm oil-derived tocotrienol rich fraction (palm TRF) supplementation on NQO1 gene and protein expression in mice livers. Western blot and qPCR assays were used to detect NQO1 expression levels. It was found that palm TRF significantly induced NQO1 expression at all doses given. In conclusion, palm TRF treatment increased NQO1 gene and protein expression in mice liver dose dependently, with the highest expression seen in mice treated with 1000 mg/kg palm TRF, followed by 500 and 200 mg/kg respectively.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalJournal of Applied Pharmaceutical Science
Volume6
Issue number8
DOIs
Publication statusPublished - 2016

Fingerprint

Tocotrienols
NAD
Oxidoreductases
Liver
Hydroquinones
Gene Expression
Quinones
Poisons
Proteins
Western Blotting
benzoquinone
palm oil
Diet
Health

Keywords

  • Gene expression
  • NQO1, palm oil
  • Tocotrienol rich fraction
  • Vitamin E

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medicine (miscellaneous)
  • Pharmacology (medical)

Cite this

Tocotrienol-rich palm oil extract induces NAD(P)H : Quinone Oxidoreductase 1 (NQO1) expression in mice liver. / Atia, Ahmed; Alrawaiq, Nadia; Abdullah, Azman.

In: Journal of Applied Pharmaceutical Science, Vol. 6, No. 8, 2016, p. 127-134.

Research output: Contribution to journalArticle

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AU - Abdullah, Azman

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