Tocotrienol rich fraction (TRF) supplementation protects against oxidative DNA damage and improves cognitive functions in Wistar rats

N. M. Taridi, Mohamad Fairuz Yahaya, Seong Lin Teoh, A. A. Latiff, W. Z. Wan Ngah, Srijit Das, M. Mazlan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim: Oxidative stress is caused by imbalance between the productions of reactive oxygen species (ROS) and antioxidant defense mechanisms. Palm oil antioxidants such as tocotrienol rich fraction (TRF) is known to have neuroprotective effects on neurones by acting against free radical induced neuronal cell death. This study was undertaken to elucidate the effect of TRF on oxidative DNA damage and cognitive functions in experimental rats. Materials and Methods: A total of 20 male Wistar rats (aged 3 months) were divided into 2 groups: (i) control group fed with distilled water and (ii) experimental group fed with TRF (200 mg/kg body weight) for 8 months. DNA damage was determined using Comet assay. Antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were assessed in the blood. The Morris Water Maze (MWM) test was used to evaluate the cognitive functions. Results: DNA damage was significantly reduced in the experimental group supplemented with TRF compared to the control group (p <0.05). In the group supplemented with TRF, the percentage of DNA damage was 2.87 ± 0.48% compared to 5.96 ± 0.43% in the control group. SOD, GPx, and CAT enzyme activities increased in experimental group. Results from MWM showed improvement in cognitive functions as determined by latency to target platform, swim path and average speed between TRF and control groups. Conclusions: Continuous supplementation of TRF for 8 months reduced DNA damage and exhibited positive influence in spatial learning and memory.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalClinica Terapeutica
Volume162
Issue number2
Publication statusPublished - 2011

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Tocotrienols
Cognition
DNA Damage
Wistar Rats
Control Groups
Antioxidants
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Water
Comet Assay
Neuroprotective Agents
Defense Mechanisms
Enzymes
Free Radicals
Reactive Oxygen Species
Oxidative Stress
Cell Death
Body Weight
Neurons

Keywords

  • Aging
  • Brain
  • Cognitive function
  • Oxidative stress
  • TRF

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tocotrienol rich fraction (TRF) supplementation protects against oxidative DNA damage and improves cognitive functions in Wistar rats. / Taridi, N. M.; Yahaya, Mohamad Fairuz; Teoh, Seong Lin; Latiff, A. A.; Wan Ngah, W. Z.; Das, Srijit; Mazlan, M.

In: Clinica Terapeutica, Vol. 162, No. 2, 2011, p. 93-98.

Research output: Contribution to journalArticle

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AU - Latiff, A. A.

AU - Wan Ngah, W. Z.

AU - Das, Srijit

AU - Mazlan, M.

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