Tocotrienol rich fraction prevents fenitrothion induced pancreatic damage by restoring antioxidant status

Siti Balkis Budin, Cha Ming Han, Putri Ayu Jayusman, Taib Izatus Shima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fenitrothion (FNT) is extensively used as pesticide and may induce oxidative stress in various organs. Tocotrienol, a form of vitamin E found in palm oil, reduces oxidative impairments in pathological conditions. This study aims to investigate the effects of palm oil tocotrienol rich fraction (TRF) on fenitrothion-induced oxidative damage in rat pancreas. Forty male Sprague-Dawley rats were divided into four groups: control group, FNT group, TRF group and FNT+TRF group. Regimens FNT (20 mg kg-1 b.wt.) and TRF (200 mg kg-1 b.wt.) were force-fed for 28 consecutive days with control group only receiving corn oil. Chronic administration of fenitrothion significantly (p<0.05) induced oxidative damage in pancreas of rats with elevated malondialdehyde and protein carbonyl level. Depletion of glutathione and significant (p<0.05) reduction in antioxidant enzyme activities in pancreas homogenate additionally suggested induction of oxidative stress. Despite these changes in pancreas of intoxicated rats, no significant (p<0.05) changes in blood glucose and pancreas histology were observed. Co-administration of FNT with TRF alleviated these oxidative changes and significantly (p<0.05) restored antioxidant status. Enzymatic activities of Superoxide Dismutase (SOD) and Catalase (CAT) were normalized. In conclusion, tocotrienol rich fraction of palm oil prevents fenitrothion-induced pancreatic oxidative damage in rats.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume15
Issue number11
DOIs
Publication statusPublished - 2012

Fingerprint

fenitrothion
tocotrienols
antioxidants
pancreas
palm oils
rats
oxidative stress
corn oil
malondialdehyde
blood glucose
histology
vitamin E
glutathione
catalase
superoxide dismutase
pesticides
enzyme activity

Keywords

  • Antioxidants
  • Blood glucose
  • Lipid peroxidation
  • Organophosphate
  • Pancreas

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Tocotrienol rich fraction prevents fenitrothion induced pancreatic damage by restoring antioxidant status. / Budin, Siti Balkis; Han, Cha Ming; Jayusman, Putri Ayu; Izatus Shima, Taib.

In: Pakistan Journal of Biological Sciences, Vol. 15, No. 11, 2012, p. 517-523.

Research output: Contribution to journalArticle

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