Tocotrienol-rich fraction prevents cell cycle arrest and elongates telomere length in senescent human diploid fibroblasts

Suzana Makpol, Lina Wati Durani, Chua Kien Hui, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This study determined the molecular mechanisms of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs). Primary culture of HDFs at various passages were incubated with 0.5mg/mL TRF for 24 h. Telomere shortening with decreased telomerase activity was observed in senescent HDFs while the levels of damaged DNA and number of cells in G0/G1 phase were increased and S phase cells were decreased. Incubation with TRF reversed the morphology of senescent HDFs to resemble that of young cells with decreased activity of SA - gal, damaged DNA, and cells in G0/G1 phase while cells in the S phase were increased. Elongated telomere length and restoration of telomerase activity were observed in TRF-treated senescent HDFs. These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence.

Original languageEnglish
Article number506171
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Tocotrienols
Telomere
Fibroblasts
Cell Cycle Checkpoints
Diploidy
Cells
Telomerase
Cell Cycle Resting Phase
Cell Aging
G1 Phase
S Phase
DNA
Telomere Shortening
Cell culture
Restoration
Cell Count
Aging of materials

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Tocotrienol-rich fraction prevents cell cycle arrest and elongates telomere length in senescent human diploid fibroblasts. / Makpol, Suzana; Durani, Lina Wati; Kien Hui, Chua; Mohd Yusof, Yasmin Anum; Wan Ngah, Wan Zurinah.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 506171, 2011.

Research output: Contribution to journalArticle

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