Tocotrienol-rich fraction of palm oil reduced pancreatic damage and oxidative stress in streptozotocin-induced diabetic rats

Siti Balkis Budin, Khairunnisa' Md Yusof, Muhd Hanis Md Idris, Zariyantey Abd Hamid, Jamaludin Mohamed

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Abstract

Increased free radicals production and decreased of antioxidant defense system are common complications encounter in diabetes mellitus (DM). Tocotrienol-rich fraction of palm oil (TRF) is an antioxidant that is rich in tocotrienol and tocopherol. The main aim of the present study was to determine the effect of TRF against oxidative stress in pancreas of diabetic rats. Forty male Sprague Dawley rats were divided into four groups: non-diabetic (NDM), non-diabetic supplemented TRF (NDM+TRF), diabetic rats and diabetic rats supplemented TRF (diabetic+TRF). Diabetes was induced through a single intravenous injection of streptozotocin at the dose of 45 mg/kg and TRF was administered orally and daily at the dose of 200 mg/kg for 28 days. At the end of four weeks study, TRF supplementation reduced hyperglycemic state and prevented the severe damage to the islet cells of pancreas. Supplemented TRF also significantly reduced (p<0.05) the status of oxidative stress of pancreas as evidenced by reduction in the level of malondealdehyde (MDA) and protein carbonyl than those diabetic rats alone. Meanwhile, improved antioxidant defense system was also observed in diabetic group supplemented with TRF which was demonstrated by significantly higher (p<0.05) levels of glutathione (GSH) as well as superoxide dismutase (SOD) activities as compared to diabetes rats without TRF supplementation. In conclusion, TRF was able to reduce pancreatic damage and oxidative stress in streptozotocin-induced diabetic rats and the process was probably mediated by its antioxidant effects.

Original languageEnglish
Pages (from-to)2367-2374
Number of pages8
JournalAustralian Journal of Basic and Applied Sciences
Volume5
Issue number12
Publication statusPublished - Dec 2011

Fingerprint

Tocotrienols
Streptozocin
Oxidative Stress
Antioxidants
Pancreas
Tocopherols
Islets of Langerhans
Intravenous Injections
Superoxide Dismutase
Free Radicals
Glutathione
Sprague Dawley Rats
palm oil
Diabetes Mellitus

Keywords

  • Antioxidants
  • Histology
  • Lipid peroxidation
  • Protein oxidation
  • Type 1 diabetes mellitus

ASJC Scopus subject areas

  • General

Cite this

Tocotrienol-rich fraction of palm oil reduced pancreatic damage and oxidative stress in streptozotocin-induced diabetic rats. / Budin, Siti Balkis; Yusof, Khairunnisa' Md; Idris, Muhd Hanis Md; Abd Hamid, Zariyantey; Mohamed, Jamaludin.

In: Australian Journal of Basic and Applied Sciences, Vol. 5, No. 12, 12.2011, p. 2367-2374.

Research output: Contribution to journalArticle

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