Tocotrienol-rich fraction modulates genes expression in oxidative stress-induced Caenorhabditis e legans

Goon Jo Aan, Noralisa Abdul Karim, Mohd Shahril Aszrin Zainudin, Suzana Makpol

Research output: Contribution to journalArticle

Abstract

Objective: The study was done to determine the effect of Tocotrienol rich fraction (TRF) on the expression of RNAs in C. elegans under oxidative stress. Methods: The nematodes were divided into 4 groups and treated accordingly: control; TRF; hydrogen peroxide (H2O2); TRF treatment before and after H2O2-induction (TRF+H2O2+TRF). Expressions of RNAs were analyzed with Affymetrix Genechip C. elegans Genome Array and Genespring GX11 software where differentially expressed genes were further analyzed using gene ontology (GO). Selected genes (unc-15, cit-1.2, ftn-1, rsks-1, unc-4 and daf-12) were analyzed with RT-qPCR to validate the results. Results: TRF modulated the expression of 314 genes involved in determination of adult lifespan, regulation of growth and lipid modification. A total of 440 genes involved in RNA metabolic processes, transcription, growth and differentiation of muscle and nerve cells were differently expressed following H2O2 induction. TRF treatment before and after H2O2- induction resulted in 438 differentially expressed genes involved in RNA metabolic processes, transcription, response to xenobiotic stimulus and protein amino acid phosphorylation. Conclusion: TRF modulates the expression of genes involved in the regulation of lifespan in C. elegans.

Original languageEnglish
Pages (from-to)711-721
Number of pages11
JournalBangladesh Journal of Medical Science
Volume18
Issue number4
DOIs
Publication statusPublished - 9 Sep 2019

Fingerprint

Caenorhabditis
Tocotrienols
Oxidative Stress
Gene Expression
RNA
Genes
Gene Ontology
Xenobiotics
Growth
Muscle Cells
Hydrogen Peroxide
Software
Phosphorylation
Genome
Lipids
Neurons
Amino Acids

Keywords

  • Antioxidant
  • C.elegans
  • Oxidative stress
  • Tocotrienol

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tocotrienol-rich fraction modulates genes expression in oxidative stress-induced Caenorhabditis e legans. / Aan, Goon Jo; Abdul Karim, Noralisa; Zainudin, Mohd Shahril Aszrin; Makpol, Suzana.

In: Bangladesh Journal of Medical Science, Vol. 18, No. 4, 09.09.2019, p. 711-721.

Research output: Contribution to journalArticle

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