Tocotrienol-rich fraction from palm oil prevents oxidative damage in diabetic rats

Fatmah A. Matough, Siti Balkis Budin, Zariyantey Abd Hamid, Mariati Abdul Rahman, Nasar Al-Wahaibi, Jamaludin Mohamed

Research output: Contribution to journalArticle

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Abstract

Objectives: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. Methods: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. Results: FBG levels for the STZ-induced diabetic rats were significantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also significantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabetic rats when compared to the control group and diabetic group with TRF. The results of the superoxide dismutase and glutathione peroxidase activities were significantly lower in the STZ-induced diabetic rats than in the normal group (P <0.001). The levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were significantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabetic rats treated with TRF. Conclusion: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabetic rats.

Original languageEnglish
JournalSultan Qaboos University Medical Journal
Volume14
Issue number1
Publication statusPublished - Jan 2014

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Tocotrienols
Streptozocin
Oxidative Stress
Blood Glucose
Tail
Fasting
DNA
Leukocytes
Antioxidants
Erythrocytes
Glutathione Disulfide
Erythrocyte Membrane
Glutathione Peroxidase
palm oil
Malondialdehyde
Lipid Peroxidation
Superoxide Dismutase
Glutathione
Biomarkers
Control Groups

Keywords

  • Antioxidants
  • Diabetes mellitus
  • Oxidative stress
  • Tocotrienols

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tocotrienol-rich fraction from palm oil prevents oxidative damage in diabetic rats. / Matough, Fatmah A.; Budin, Siti Balkis; Abd Hamid, Zariyantey; Abdul Rahman, Mariati; Al-Wahaibi, Nasar; Mohamed, Jamaludin.

In: Sultan Qaboos University Medical Journal, Vol. 14, No. 1, 01.2014.

Research output: Contribution to journalArticle

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