Tocotrienol rich fraction and piper betle extract enhanced the viability of human peripheral blood mononuclear cells

Amirah Abdul Rahman, Norwahidah Abdul Karim, Noor Aini Abdul Hamid, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

Abstract

Objective: Tocotrienols are isomers of vitamin E, reported to have immune boosting and antiproliferative functions while Piper betle (PB) was shown to possess anti-inflammatory and antioxidant properties. The effect of tocotrienol rich fraction 70% (TRF70) and PB on PHA non-stimulated human peripheral blood mononuclear cells (PBMC) cultures is less known. The current aim is to investigate the protective or cytotoxicity potentials TRF70 and PB and correlate it with the antioxidant capacity values. Design: Free radical scavenging activities of hot water extract (HWE) of PB and TRF70 were determined by using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical trapping method. Cytotoxicity assay were performed by using 3-(4,5-dimethylthiazol-2- yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Results: TRF70 has the highest radical scavenging activity followed by PB when compared to standard antioxidants such as butylated hydroxyl toluene (BHT) and L-Ascorbic Acid (vitamin C). Treatment with TRF70 significantly (p < 0.01) increased PBMC cell viability in a dose-response manner of 5, 10, 15, 20, 25 μg/ml whereas PB showed a significant increase of cell viability at the concentration of 10, 20, 25, 50 μg/ml though this extract was cytotoxic at higher concentrations. Conclusion: Analysis revealed an increase of maximum cell viability at low concentrations of 20 μg/ml and 50 μg/ml for TRF70 and PB respectively, but at higher concentrations, a decrease of viable cells were seen.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalInternational Medical Journal
Volume20
Issue number2
Publication statusPublished - Apr 2013

Fingerprint

Piper betle
Tocotrienols
Blood Cells
Cell Survival
Antioxidants
Ascorbic Acid
Toluene
Vitamin E
Hydroxyl Radical
Free Radicals
Anti-Inflammatory Agents
Cell Culture Techniques

Keywords

  • Cell viability
  • DPPH assay
  • Peripheral blood mononuclear cells
  • Piper betle
  • Tocotrienol rich fraction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tocotrienol rich fraction and piper betle extract enhanced the viability of human peripheral blood mononuclear cells. / Abdul Rahman, Amirah; Abdul Karim, Norwahidah; Abdul Hamid, Noor Aini; Wan Ngah, Wan Zurinah.

In: International Medical Journal, Vol. 20, No. 2, 04.2013, p. 163-166.

Research output: Contribution to journalArticle

Abdul Rahman, Amirah ; Abdul Karim, Norwahidah ; Abdul Hamid, Noor Aini ; Wan Ngah, Wan Zurinah. / Tocotrienol rich fraction and piper betle extract enhanced the viability of human peripheral blood mononuclear cells. In: International Medical Journal. 2013 ; Vol. 20, No. 2. pp. 163-166.
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AU - Wan Ngah, Wan Zurinah

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N2 - Objective: Tocotrienols are isomers of vitamin E, reported to have immune boosting and antiproliferative functions while Piper betle (PB) was shown to possess anti-inflammatory and antioxidant properties. The effect of tocotrienol rich fraction 70% (TRF70) and PB on PHA non-stimulated human peripheral blood mononuclear cells (PBMC) cultures is less known. The current aim is to investigate the protective or cytotoxicity potentials TRF70 and PB and correlate it with the antioxidant capacity values. Design: Free radical scavenging activities of hot water extract (HWE) of PB and TRF70 were determined by using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical trapping method. Cytotoxicity assay were performed by using 3-(4,5-dimethylthiazol-2- yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Results: TRF70 has the highest radical scavenging activity followed by PB when compared to standard antioxidants such as butylated hydroxyl toluene (BHT) and L-Ascorbic Acid (vitamin C). Treatment with TRF70 significantly (p < 0.01) increased PBMC cell viability in a dose-response manner of 5, 10, 15, 20, 25 μg/ml whereas PB showed a significant increase of cell viability at the concentration of 10, 20, 25, 50 μg/ml though this extract was cytotoxic at higher concentrations. Conclusion: Analysis revealed an increase of maximum cell viability at low concentrations of 20 μg/ml and 50 μg/ml for TRF70 and PB respectively, but at higher concentrations, a decrease of viable cells were seen.

AB - Objective: Tocotrienols are isomers of vitamin E, reported to have immune boosting and antiproliferative functions while Piper betle (PB) was shown to possess anti-inflammatory and antioxidant properties. The effect of tocotrienol rich fraction 70% (TRF70) and PB on PHA non-stimulated human peripheral blood mononuclear cells (PBMC) cultures is less known. The current aim is to investigate the protective or cytotoxicity potentials TRF70 and PB and correlate it with the antioxidant capacity values. Design: Free radical scavenging activities of hot water extract (HWE) of PB and TRF70 were determined by using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical trapping method. Cytotoxicity assay were performed by using 3-(4,5-dimethylthiazol-2- yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Results: TRF70 has the highest radical scavenging activity followed by PB when compared to standard antioxidants such as butylated hydroxyl toluene (BHT) and L-Ascorbic Acid (vitamin C). Treatment with TRF70 significantly (p < 0.01) increased PBMC cell viability in a dose-response manner of 5, 10, 15, 20, 25 μg/ml whereas PB showed a significant increase of cell viability at the concentration of 10, 20, 25, 50 μg/ml though this extract was cytotoxic at higher concentrations. Conclusion: Analysis revealed an increase of maximum cell viability at low concentrations of 20 μg/ml and 50 μg/ml for TRF70 and PB respectively, but at higher concentrations, a decrease of viable cells were seen.

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