Tocotrienol and tocopherol were protective against xanthine plus xanthine oxidase induced oxidative stress

Kamisah Yusof, A. Adam, W. Z. Wan Ngah, A. Gapor, B. A K Khalid, A. Marzuki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The free radical generator, xanthine (X) plus xanthine oxidase (XO) was utilised to produce oxidative stress in vivo. Protection against this condition by tocotrienol (80% γ isomer) and α-tocopherol acetate was investigated. Rats were given two doses of either one of the vitamin E (100 mgkg-1day-1, i.p., 24 h interval between doses) prior to exposure to X (0.23 mgkg-1, i.v.) and XO (3 Ukg-1, iv). Creatine phosphokinase (CPK), aspartate transaminase (AST) and alkaline phosphatase (ALP) were determined in the plasma. Rat organs were sampled for the determination of thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation, and for antioxidants such as reduced glutathione (GSH) and superoxide dismutase (SOD), and for oxidized glutathione (GSSG). X+XO significantly increased plasma CPK levels at 0.5, 1 and 2h. Both tocotrienol and α-tocopherol prevented the X+XO-induced increases in CPK levels (p<0.05). AST and ALP were unaffected by the radical generator. X+XO at 1h significantly increased TBARS in the heart and lung without affecting that of the liver or kidney. In rats pretreated with both forms of vitamin E, heart TBARS were significantly lower than in the nonpretreated rats. Lung TBARS remained unaffected by vitamin E pretreatment. Neither SOD nor GSH and GSSG were affected by X+XO. These results suggest that the heart and lungs are susceptible to oxidative damage caused by X+XO. Administration of α-tocopherol or tocotrienol prior to exposure to X+XO protected the heart against oxidative stress. There was no difference in the extent of protection afforded by these two forms of vitamin E.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalAsia Pacific Journal of Pharmacology
Volume14
Issue number4
Publication statusPublished - 2000

Fingerprint

Tocotrienols
Xanthine
Tocopherols
Xanthine Oxidase
Oxidative Stress
Thiobarbituric Acid Reactive Substances
Vitamin E
Glutathione Disulfide
Creatine Kinase
Aspartate Aminotransferases
Lung
Superoxide Dismutase
Alkaline Phosphatase
alpha-Tocopherol
Lipid Peroxidation
Free Radicals
Glutathione
Antioxidants
Kidney

Keywords

  • Oxidative stress
  • Tocopherol
  • Tocotrienol
  • Xanthine plus xanthine oxidase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Tocotrienol and tocopherol were protective against xanthine plus xanthine oxidase induced oxidative stress. / Yusof, Kamisah; Adam, A.; Wan Ngah, W. Z.; Gapor, A.; Khalid, B. A K; Marzuki, A.

In: Asia Pacific Journal of Pharmacology, Vol. 14, No. 4, 2000, p. 111-116.

Research output: Contribution to journalArticle

Yusof, K, Adam, A, Wan Ngah, WZ, Gapor, A, Khalid, BAK & Marzuki, A 2000, 'Tocotrienol and tocopherol were protective against xanthine plus xanthine oxidase induced oxidative stress', Asia Pacific Journal of Pharmacology, vol. 14, no. 4, pp. 111-116.
Yusof, Kamisah ; Adam, A. ; Wan Ngah, W. Z. ; Gapor, A. ; Khalid, B. A K ; Marzuki, A. / Tocotrienol and tocopherol were protective against xanthine plus xanthine oxidase induced oxidative stress. In: Asia Pacific Journal of Pharmacology. 2000 ; Vol. 14, No. 4. pp. 111-116.
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