Titanium mesh with expanded respiratory epithelial cells in tracheal reconstruction

Ruszymah Bt Hj Idrus, Nur Adelina Ahmad Noruddin, Ruszymah Idrus, Chen Hui Cheng, Lokanathan Yogeswaran, Aminuddin Bin Saim, Aminuddin Bin Saim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The objective of this study was to treat surgically induced tracheal defect in sheep with hybrid bilayered trachea constructed using autologous nasal respiratory epithelial cells and fibroblast seeded on titanium scaffold. Twelve 3 to 6 month-old male sheep were used in this study. Nasal turbinate was harvested and digested with Collagenase type I and cultured using co-cultured technique. Upon confluence, the cells were trypsinised and reconstructed into bilayered tissue with autologous fibrin. Under general anesthesia, a 2×3 cm sheep tracheal defect was created and the construct with titanium mesh was placed on the defect. The defect in the control group was only closed with the titanium mesh and fibrin without cells. At four weeks post transplantation, none of the sheep in the control group (n = 6) survived. In the study group (n = 6), gross observation of the implanted area showed slight narrowing of the lumen but the inner lining were smooth with evidence of epithelial regeneration. Presence of PKH26 stained cells showed the viability of nasal respiratory cells in the implanted construct. This study was a proof-of-concept that hybrid bi-layered trachea constructed using autologous nasal respiratory epithelial cells and fibroblast seeded on titanium scaffold was able to treat a surgically induced partial segmental tracheal defect in a big animal model.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalJournal of Biomaterials and Tissue Engineering
Volume4
Issue number5
DOIs
Publication statusPublished - 1 May 2014

Fingerprint

Titanium
Nose
Sheep
Epithelial Cells
Defects
Trachea
Fibrin
Fibroblasts
Scaffolds
Turbinates
Control Groups
Collagenases
General Anesthesia
Regeneration
Cell Survival
Linings
Animal Models
Transplantation
Observation
Animals

Keywords

  • Bilayered trachea mucosa
  • Sheep model airway reconstruction
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)

Cite this

Titanium mesh with expanded respiratory epithelial cells in tracheal reconstruction. / Idrus, Ruszymah Bt Hj; Noruddin, Nur Adelina Ahmad; Idrus, Ruszymah; Cheng, Chen Hui; Yogeswaran, Lokanathan; Saim, Aminuddin Bin; Saim, Aminuddin Bin.

In: Journal of Biomaterials and Tissue Engineering, Vol. 4, No. 5, 01.05.2014, p. 367-372.

Research output: Contribution to journalArticle

Idrus, Ruszymah Bt Hj ; Noruddin, Nur Adelina Ahmad ; Idrus, Ruszymah ; Cheng, Chen Hui ; Yogeswaran, Lokanathan ; Saim, Aminuddin Bin ; Saim, Aminuddin Bin. / Titanium mesh with expanded respiratory epithelial cells in tracheal reconstruction. In: Journal of Biomaterials and Tissue Engineering. 2014 ; Vol. 4, No. 5. pp. 367-372.
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