Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds

Wanyao Xia, Wei Liu, Lei Cui, Yuanchun Liu, Wei Zhong, Deli Liu, Juanjuan Wu, Chua Kien Hui, Yilin Cao

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Chitosan has been shown to be a promising scaffold for various applications in tissue engineering. In this study, a chitosan-gelatin complex was fabricated as a scaffold by a freezing and lyophilizing technique. Chitosan's structure and characteristics are similar to those of glycosaminoglycan (GAG) and its analogs, and possesses various biological activities, whereas gelatin can serve as a substrate for cell adhesion, differentiation, and proliferation. With the use of autologous chondrocytes isolated from pig's auricular cartilage and seeded onto the chitosan-gelatin scaffold, elastic cartilages have been successfully engineered at the porcine abdomen subcutaneous tissue. After 16 weeks of implantation, the engineered elastic cartilages have acquired not only normal histological and biochemical, but also mechanical properties. The tissue sections of the engineered elastic cartilages showed that the chondrocytes were enclosed in the lacuna, similar to that of native cartilage. The presence of elastic fibers in the engineered cartilages was also demonstrated by Vehoeff's staining, and immunohistochemical staining confirmed the presence of type II collagen in the engineered cartilages. Quantitatively, the GAG in the engineered cartilages reached 90% of the concentration in native auricular cartilage. Furthermore, biomechanical analysis demonstrated that the extrinsic stiffness of the engineered cartilages reached 85% of the level in native auricular cartilage when it was harvested at 16 weeks. Thus, this study demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for cartilage tissue engineering.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume71
Issue number2
DOIs
Publication statusPublished - 15 Nov 2004

Fingerprint

Chitosan
Cartilage
Gelatin
Tissue engineering
Scaffolds
Scaffolds (biology)
Glycosaminoglycans
Tissue
Collagen Type II
Cell adhesion
Bioactivity
Collagen
Freezing
Stiffness

Keywords

  • Cartilage
  • Chitosan
  • Gelatin
  • Scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds. / Xia, Wanyao; Liu, Wei; Cui, Lei; Liu, Yuanchun; Zhong, Wei; Liu, Deli; Wu, Juanjuan; Kien Hui, Chua; Cao, Yilin.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 71, No. 2, 15.11.2004, p. 373-380.

Research output: Contribution to journalArticle

Xia, Wanyao ; Liu, Wei ; Cui, Lei ; Liu, Yuanchun ; Zhong, Wei ; Liu, Deli ; Wu, Juanjuan ; Kien Hui, Chua ; Cao, Yilin. / Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2004 ; Vol. 71, No. 2. pp. 373-380.
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