Time and dose-dependent effects of Labisia pumila on the bone strength of postmenopausal osteoporosis rat model

Nadia Mohd Effendy, Shahrum Abdullah, Mohd Faridz Mod Yunoh, Ahmad Nazrun Shuid

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Post-menopausal osteoporosis has long been treated and prevented by estrogen replacement therapy (ERT). Despite its effectiveness, ERT is associated with serious adverse effects. Labisia pumila var. alata (LP) is a herb with potential as an alternative agent to ERT due to its phytoestrogenic, antioxidative and anti-inflammatory effects on bone. This study aimed to determine the effects of LP supplementation on bone biomechanical strength of postmenopausal osteoporosis rat model. Methods: Ninety-six female Sprague-Dawley rats aged 4 to 5months old were randomly divided into six groups; six rats in the baseline group (BL) and eighteen rats in each group of; Sham- operated (Sham), ovariectomised control (OVXC) and ovariectomised with daily oral gavages of Premarin at 64.5μg/kg (ERT), LP at 20mg/kg (LP20) and LP at 100mg/kg (LP100) respectively. These groups were subdivided into three, six and nine weeks of treatment periods. Rats in BL group were euthanized before the start of the study, while other rats were euthanized after completion of their treatments. Femora were dissected out for biomechanical strength analysis using Instron Universal Model 5848 Micro Tester. Results: OVXC group showed deterioration in the bone biomechanical strength with time. Both ERT and LP supplemented rats showed improvements in bone strength parameters such as maximum load, displacement, stiffness, stress, and Young Modulus. The most improved bone strength was seen in rats given LP at the dose of 100mg/kg for nine weeks. Conclusion: LP supplementation at 100mg/kg was more effective than ERT in reversing ovariectomy-induced bone biomechanical changes.

Original languageEnglish
Article number58
JournalBMC Complementary and Alternative Medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 12 Mar 2015

Fingerprint

Postmenopausal Osteoporosis
Estrogen Replacement Therapy
Bone and Bones
Conjugated (USP) Estrogens
Elastic Modulus
Ovariectomy
Femur
Sprague Dawley Rats
Anti-Inflammatory Agents
Therapeutics

Keywords

  • Biomechanical
  • Bone strength
  • Estrogen
  • Osteoporosis
  • Postmenopausal

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Time and dose-dependent effects of Labisia pumila on the bone strength of postmenopausal osteoporosis rat model. / Mohd Effendy, Nadia; Abdullah, Shahrum; Yunoh, Mohd Faridz Mod; Shuid, Ahmad Nazrun.

In: BMC Complementary and Alternative Medicine, Vol. 15, No. 1, 58, 12.03.2015.

Research output: Contribution to journalArticle

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