Tiger milk mushroom cultivation by using submerged culture technique

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3 Citations (Scopus)

Abstract

The aim of this project is to study the growth of tiger milk mushroom or Lignosus rhinocerus by using submerged culture technique. The strains used in this study are L. rhinocerus AOI and L. rhinocerus 7-19 but for growth kinetic only strain of L. rhinocerus 7-19 is studied by using Monod model. Based on the Monod model, parameters such as specific growth rate, μ and Monod coefficient, Ks can be determined. Value for μmax is 0.54 hr-1 and value for Ks is 0.29 g/L. Doubling time, td is 22 minutes and 25 seconds, and biomass yield coefficient, Y X/S is 0.25. The optimum growth conditions for L. rhinocerus 7-19 are studied by using 6 types of carbon sources and 6 types of nitrogen sources. The data show that the highest growth is obtained by using lactose, followed by xylose, maltose, glucose, sucrose and fructose. Lactose shows the highest growth rate because of high percentage of carbon content. Besides, the biomass growth also high for xylose, due to xilose is a type of wood sugar and factor of mushroom habitat. The study of nitrogen sources show that the usage of ammonium salts such as NH4Cl, NH4NO3 and (NH4)2HPO4 can hinder the growth of biomass. The usage of other nitrogen sources such as meat peptone, yeast extract and peptone can increase the growth of biomass. Thus, the presence of carbon source and nitrogen source are crucial to biomass growth for both strains.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalWSEAS Transactions on Biology and Biomedicine
Volume9
Issue number3
Publication statusPublished - Apr 2012

Fingerprint

Pleurotus tuber-regium
Tigers
mushroom growing
Culture Techniques
Agaricales
Milk
Biomass
biomass
Growth
Nitrogen
peptones
nitrogen
xylose
lactose
Peptones
carbon
Carbon
Xylose
Lactose
ammonium salts

Keywords

  • β-glucan
  • Biomass
  • Lignosus rhinocerus
  • Monod model
  • Nitrogen sources

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

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title = "Tiger milk mushroom cultivation by using submerged culture technique",
abstract = "The aim of this project is to study the growth of tiger milk mushroom or Lignosus rhinocerus by using submerged culture technique. The strains used in this study are L. rhinocerus AOI and L. rhinocerus 7-19 but for growth kinetic only strain of L. rhinocerus 7-19 is studied by using Monod model. Based on the Monod model, parameters such as specific growth rate, μ and Monod coefficient, Ks can be determined. Value for μmax is 0.54 hr-1 and value for Ks is 0.29 g/L. Doubling time, td is 22 minutes and 25 seconds, and biomass yield coefficient, Y X/S is 0.25. The optimum growth conditions for L. rhinocerus 7-19 are studied by using 6 types of carbon sources and 6 types of nitrogen sources. The data show that the highest growth is obtained by using lactose, followed by xylose, maltose, glucose, sucrose and fructose. Lactose shows the highest growth rate because of high percentage of carbon content. Besides, the biomass growth also high for xylose, due to xilose is a type of wood sugar and factor of mushroom habitat. The study of nitrogen sources show that the usage of ammonium salts such as NH4Cl, NH4NO3 and (NH4)2HPO4 can hinder the growth of biomass. The usage of other nitrogen sources such as meat peptone, yeast extract and peptone can increase the growth of biomass. Thus, the presence of carbon source and nitrogen source are crucial to biomass growth for both strains.",
keywords = "β-glucan, Biomass, Lignosus rhinocerus, Monod model, Nitrogen sources",
author = "{Abd Rahman}, Norliza and Fauzi Daud and Kalil, {Mohd. Sahaid} and S. Ahmad",
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AU - Abd Rahman, Norliza

AU - Daud, Fauzi

AU - Kalil, Mohd. Sahaid

AU - Ahmad, S.

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N2 - The aim of this project is to study the growth of tiger milk mushroom or Lignosus rhinocerus by using submerged culture technique. The strains used in this study are L. rhinocerus AOI and L. rhinocerus 7-19 but for growth kinetic only strain of L. rhinocerus 7-19 is studied by using Monod model. Based on the Monod model, parameters such as specific growth rate, μ and Monod coefficient, Ks can be determined. Value for μmax is 0.54 hr-1 and value for Ks is 0.29 g/L. Doubling time, td is 22 minutes and 25 seconds, and biomass yield coefficient, Y X/S is 0.25. The optimum growth conditions for L. rhinocerus 7-19 are studied by using 6 types of carbon sources and 6 types of nitrogen sources. The data show that the highest growth is obtained by using lactose, followed by xylose, maltose, glucose, sucrose and fructose. Lactose shows the highest growth rate because of high percentage of carbon content. Besides, the biomass growth also high for xylose, due to xilose is a type of wood sugar and factor of mushroom habitat. The study of nitrogen sources show that the usage of ammonium salts such as NH4Cl, NH4NO3 and (NH4)2HPO4 can hinder the growth of biomass. The usage of other nitrogen sources such as meat peptone, yeast extract and peptone can increase the growth of biomass. Thus, the presence of carbon source and nitrogen source are crucial to biomass growth for both strains.

AB - The aim of this project is to study the growth of tiger milk mushroom or Lignosus rhinocerus by using submerged culture technique. The strains used in this study are L. rhinocerus AOI and L. rhinocerus 7-19 but for growth kinetic only strain of L. rhinocerus 7-19 is studied by using Monod model. Based on the Monod model, parameters such as specific growth rate, μ and Monod coefficient, Ks can be determined. Value for μmax is 0.54 hr-1 and value for Ks is 0.29 g/L. Doubling time, td is 22 minutes and 25 seconds, and biomass yield coefficient, Y X/S is 0.25. The optimum growth conditions for L. rhinocerus 7-19 are studied by using 6 types of carbon sources and 6 types of nitrogen sources. The data show that the highest growth is obtained by using lactose, followed by xylose, maltose, glucose, sucrose and fructose. Lactose shows the highest growth rate because of high percentage of carbon content. Besides, the biomass growth also high for xylose, due to xilose is a type of wood sugar and factor of mushroom habitat. The study of nitrogen sources show that the usage of ammonium salts such as NH4Cl, NH4NO3 and (NH4)2HPO4 can hinder the growth of biomass. The usage of other nitrogen sources such as meat peptone, yeast extract and peptone can increase the growth of biomass. Thus, the presence of carbon source and nitrogen source are crucial to biomass growth for both strains.

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