Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons

Norsharina Ismail, Maznah Ismail, Musalmah Mazlan, Latiffah Abdul Latiff, Mustapha Umar Imam, Shahid Iqbal, Nur Hanisah Azmi, Siti Aisyah Abd Ghafar, Kim Wei Chan

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1-40 sequence (Aβ1-40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aβ1-40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 μM) and subsequent exposure to 10 μM Aβ1-40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aβ1-40 alone. TQ pretreatment inhibited Aβ1-40- induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aβ1-40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer's disease.

Original languageEnglish
Pages (from-to)1159-1169
Number of pages11
JournalCellular and Molecular Neurobiology
Volume33
Issue number8
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Amyloid
Neurons
Nigella sativa
Apoptosis
Caspase 8
Neurites
Caspases
thymoquinone
Caspase 3
DNA Damage
Chromatin
Free Radicals
Reactive Oxygen Species
Alzheimer Disease
Cell Death
Peptides

Keywords

  • β-Amyloid
  • Alzheimer's disease
  • Neurotoxicity
  • Primary cultured cerebellar granule neurons
  • Thymoquinone

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Ismail, N., Ismail, M., Mazlan, M., Latiff, L. A., Imam, M. U., Iqbal, S., ... Chan, K. W. (2013). Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons. Cellular and Molecular Neurobiology, 33(8), 1159-1169. https://doi.org/10.1007/s10571-013-9982-z

Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons. / Ismail, Norsharina; Ismail, Maznah; Mazlan, Musalmah; Latiff, Latiffah Abdul; Imam, Mustapha Umar; Iqbal, Shahid; Azmi, Nur Hanisah; Ghafar, Siti Aisyah Abd; Chan, Kim Wei.

In: Cellular and Molecular Neurobiology, Vol. 33, No. 8, 2013, p. 1159-1169.

Research output: Contribution to journalArticle

Ismail, N, Ismail, M, Mazlan, M, Latiff, LA, Imam, MU, Iqbal, S, Azmi, NH, Ghafar, SAA & Chan, KW 2013, 'Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons', Cellular and Molecular Neurobiology, vol. 33, no. 8, pp. 1159-1169. https://doi.org/10.1007/s10571-013-9982-z
Ismail, Norsharina ; Ismail, Maznah ; Mazlan, Musalmah ; Latiff, Latiffah Abdul ; Imam, Mustapha Umar ; Iqbal, Shahid ; Azmi, Nur Hanisah ; Ghafar, Siti Aisyah Abd ; Chan, Kim Wei. / Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons. In: Cellular and Molecular Neurobiology. 2013 ; Vol. 33, No. 8. pp. 1159-1169.
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