Three β-galactosidase cDNA clones related to fruit ripening in papaya (Carica papaya)

Roohaida Othman, Huei Li Chong, Tze Siang Choo, Zainon Mohd Ali

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

β-Galactosidase (EC 3. 2. 1. 23) is a hydrolase which plays an important role in cell wall modification and fruit softening during ripening. In this study, three full-length β-galactosidase cDNA clones were successfully obtained from papaya mesocarp using different approaches. pPGBII which is 2,771 bp in size, was isolated from a papaya ripe mesocarp cDNA library using a heterologous probe. The other two cDNA clones, pBG(a) and pBG(b), which are 3,168 and 2,580 bp in size, respectively, were amplified from ripe papaya fruit using the reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) approaches. The pPBGII, pBG(a) and pBG(b) cDNAs, respectively, were expected to yield a putative mature polypeptide of 79, 91 and 56 kDa with isoelectric points (pI) of 8. 12, 8. 75 and 8. 26. The genomic DNA gel blot analysis indicated that all of the β-galactosidase genes exist as multiple copies in the papaya genome hence they belong to a multigene family. All three cDNA clones were expressed in fruits during ripening with varying patterns. In mesocarp, pPBGII mRNA was only expressed during ripening and peaked at the half-ripe stage when the fruits undergo dramatic softening. Meanwhile, pBG(a) mRNA expression increased from the immature green stage to the half-ripe stage where it reached maximum level before declining. pBG(b) mRNA level accumulated abundantly at the mature green stage and decreased thereafter. Therefore, we suggest that pPBGII and pBG(a) cDNA clones characterized in this work may be involved in fruit softening during papaya ripening while the fruit-specific pBG(b) may be related to early ripening stage.

Original languageEnglish
Pages (from-to)2301-2310
Number of pages10
JournalActa Physiologiae Plantarum
Volume33
Issue number6
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Galactosidases
Carica
galactosidases
Carica papaya
papayas
Fruit
ripening
Complementary DNA
Clone Cells
clones
fruits
mesocarp
hydrolases
Messenger RNA
rapid amplification of cDNA ends
Isoelectric Point
Hydrolases
isoelectric point
Multigene Family
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • β-Galactosidase
  • cDNA clones
  • Papaya
  • Ripening
  • Softening

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Three β-galactosidase cDNA clones related to fruit ripening in papaya (Carica papaya). / Othman, Roohaida; Chong, Huei Li; Choo, Tze Siang; Ali, Zainon Mohd.

In: Acta Physiologiae Plantarum, Vol. 33, No. 6, 11.2011, p. 2301-2310.

Research output: Contribution to journalArticle

Othman, Roohaida ; Chong, Huei Li ; Choo, Tze Siang ; Ali, Zainon Mohd. / Three β-galactosidase cDNA clones related to fruit ripening in papaya (Carica papaya). In: Acta Physiologiae Plantarum. 2011 ; Vol. 33, No. 6. pp. 2301-2310.
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