The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering

An in vivo analysis

S. Munirah, S. H. Kim, Ruszymah Idrus, Gilson Khang

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Our preliminary results indicated that fibrin and poly(lacticco-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl 2) solution. PLGA-seeded chondrocytes were used as a control. Resulting constructs were implanted subcutaneously, at the dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and sulphated-glycosaminoglycan (sGAG) analyses were performed at each time point of 1, 2 and 4 weeks post-implantation. Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and glycosaminoglycan (GAG) in fibrin/PLGA hybrid constructs was confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene encoded cartilage-specific markers, collagen type II and aggrecan core protein. The sGAG production in fibrin/PLGA hybrid constructs was higher than in the PLGA group. In conclusion, fibrin/PLGA hybrid scaffold promotes cartilaginous tissue formation in vivo and may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalEuropean Cells and Materials
Volume15
Publication statusPublished - Jan 2008

Fingerprint

glycolic acid
Cartilage
Articular Cartilage
Tissue Engineering
Scaffolds (biology)
Fibrin
Tissue engineering
Acids
A73025
Scaffolds
Chondrocytes
Collagen Type II
Tissue
Collagen
Gene Expression
polylactic acid-polyglycolic acid copolymer
Milk
Aggrecans
Chondrogenesis
Alcian Blue

Keywords

  • Articular cartilage
  • Chondrocytes
  • Fibrin
  • Poly(lactic-co-glycolic acid)
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Biochemistry
  • Biomaterials

Cite this

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering : An in vivo analysis. / Munirah, S.; Kim, S. H.; Idrus, Ruszymah; Khang, Gilson.

In: European Cells and Materials, Vol. 15, 01.2008, p. 41-52.

Research output: Contribution to journalArticle

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