The use of bone marrow derived mesenchymal stem cell for cornea regeneration in rabbit model

Kong Yong Then, M. Azlina, A. R. Ropilah, Ruszymah Idrus, C. M. Rohaina, Min Hwei Ng

Research output: Contribution to journalArticle

Abstract

Aim or purpose: To evaluate the use of autologous bone marrow derived mesenchymal stem cells (BM-MSCs) to treat cornea stromal defect in a rabbit model. Methods: A non-randomized interventional controlled animal study involving twenty-one adult New Zealand white rabbits. Corneal deep lamellar dissections were created in three groups of rabbits and treated accordingly. Group I: Autologous bone marrow derived MSCs with autologous fibrin and human amniotic membrane. Group II: Autologous fibrin with human amniotic membrane without MSCs. Group III: No treatment. Clinical outcome was evaluated by corneal re-epithelization, corneal opacity, corneal thickness and histology. Results: BM-MSCs were successfully isolated from bone marrow of seven rabbits based on the adherence property of the cells to the plastic of the cell culture plate. At day 60 corneal thickness was significantly thicker in Group I. The localization of PKH26-labeled BM-MSCs showed an increase in cell density at the transplanted site, proving its role in cornea stromal regeneration. Although the cornea clarity was not achieved in this study, we believe that cornea stromal remodeling requires many months to years to regain its original optical quality. Conclusion: Locally transplanted BM-MSCs may be a useful source for cornea stromal regeneration. The use of autologous BM-MSCs offers a promising option for treating corneal disorder without the risk of immune-rejection and calcification.

Original languageEnglish
Pages (from-to)224-233
Number of pages10
JournalAsian Journal of Ophthalmology
Volume15
Issue number4
Publication statusPublished - 1 Jan 2017

Fingerprint

Mesenchymal Stromal Cells
Cornea
Regeneration
Bone Marrow
Rabbits
Amnion
Fibrin
Corneal Opacity
Plastics
Dissection
Histology
Cell Culture Techniques
Cell Count

Keywords

  • BMSCs
  • Corneal stroma
  • Rabbit
  • Tissue engineering
  • Transplantation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The use of bone marrow derived mesenchymal stem cell for cornea regeneration in rabbit model. / Then, Kong Yong; Azlina, M.; Ropilah, A. R.; Idrus, Ruszymah; Rohaina, C. M.; Ng, Min Hwei.

In: Asian Journal of Ophthalmology, Vol. 15, No. 4, 01.01.2017, p. 224-233.

Research output: Contribution to journalArticle

Then, Kong Yong ; Azlina, M. ; Ropilah, A. R. ; Idrus, Ruszymah ; Rohaina, C. M. ; Ng, Min Hwei. / The use of bone marrow derived mesenchymal stem cell for cornea regeneration in rabbit model. In: Asian Journal of Ophthalmology. 2017 ; Vol. 15, No. 4. pp. 224-233.
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