The use of autologous fibrin as a scaffold for cultivating autologous conjunctiva in the treatment of conjunctival defect

Safinaz Mohd Khialdin, Norzana Abd. Ghafar, M. H. Hairul Nizam, A. R. Ropilah, H. A. Faridah, Chua Kien Hui, Ruszymah Idrus, Jemaima Che Hamzah

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4 Citations (Scopus)

Abstract

The purpose of this study was to compare the use of autologous fibrin to human amniotic membrane (HAM) as a scaffold in cultivating autologous conjunctiva for transplantation in treatment of conjunctival defect. An experimental study was performed using 18 adult New Zealand white strain rabbits which were divided into 3 groups. Each group consists of 6 rabbits. The conjunctiva on the temporal site was excised to create a conjunctival epithelial defect. The excised area in the Group 1 was transplanted with autologous conjunctiva cultivated on autologous fibrin; Group 2 was transplanted with autologous conjunctiva cultivated on HAM and Group 3 was left bare. The rabbits were followed up at regular intervals until 6 weeks. The mean period of complete conjunctival epithelization was 11.50 ± 8.22 days for the autologous fibrin group, 15.33 ± 11.80 days for the HAM group and 25.33 ± 5.32 days in the bare sclera group. The epithelization rate for the autologous fibrin group was faster compared to the other two groups. However all the results were not statistically significant (p value >0.05). There were no postoperative complications noted during the follow up. Autologous fibrin is comparable to HAM as a scaffold for cultivation of conjunctiva in the treatment of conjunctival defect.

Original languageEnglish
Pages (from-to)619-626
Number of pages8
JournalCell and Tissue Banking
Volume15
Issue number4
DOIs
Publication statusPublished - 6 Nov 2014

Fingerprint

Conjunctiva
Fibrin
Scaffolds
Amnion
Membranes
Defects
Rabbits
Sclera
Autologous Transplantation

Keywords

  • Autologous transplantation
  • Conjunctiva
  • Fibrin
  • Rabbit

ASJC Scopus subject areas

  • Transplantation
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

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title = "The use of autologous fibrin as a scaffold for cultivating autologous conjunctiva in the treatment of conjunctival defect",
abstract = "The purpose of this study was to compare the use of autologous fibrin to human amniotic membrane (HAM) as a scaffold in cultivating autologous conjunctiva for transplantation in treatment of conjunctival defect. An experimental study was performed using 18 adult New Zealand white strain rabbits which were divided into 3 groups. Each group consists of 6 rabbits. The conjunctiva on the temporal site was excised to create a conjunctival epithelial defect. The excised area in the Group 1 was transplanted with autologous conjunctiva cultivated on autologous fibrin; Group 2 was transplanted with autologous conjunctiva cultivated on HAM and Group 3 was left bare. The rabbits were followed up at regular intervals until 6 weeks. The mean period of complete conjunctival epithelization was 11.50 ± 8.22 days for the autologous fibrin group, 15.33 ± 11.80 days for the HAM group and 25.33 ± 5.32 days in the bare sclera group. The epithelization rate for the autologous fibrin group was faster compared to the other two groups. However all the results were not statistically significant (p value >0.05). There were no postoperative complications noted during the follow up. Autologous fibrin is comparable to HAM as a scaffold for cultivation of conjunctiva in the treatment of conjunctival defect.",
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T1 - The use of autologous fibrin as a scaffold for cultivating autologous conjunctiva in the treatment of conjunctival defect

AU - Mohd Khialdin, Safinaz

AU - Abd. Ghafar, Norzana

AU - Hairul Nizam, M. H.

AU - Ropilah, A. R.

AU - Faridah, H. A.

AU - Kien Hui, Chua

AU - Idrus, Ruszymah

AU - Che Hamzah, Jemaima

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AB - The purpose of this study was to compare the use of autologous fibrin to human amniotic membrane (HAM) as a scaffold in cultivating autologous conjunctiva for transplantation in treatment of conjunctival defect. An experimental study was performed using 18 adult New Zealand white strain rabbits which were divided into 3 groups. Each group consists of 6 rabbits. The conjunctiva on the temporal site was excised to create a conjunctival epithelial defect. The excised area in the Group 1 was transplanted with autologous conjunctiva cultivated on autologous fibrin; Group 2 was transplanted with autologous conjunctiva cultivated on HAM and Group 3 was left bare. The rabbits were followed up at regular intervals until 6 weeks. The mean period of complete conjunctival epithelization was 11.50 ± 8.22 days for the autologous fibrin group, 15.33 ± 11.80 days for the HAM group and 25.33 ± 5.32 days in the bare sclera group. The epithelization rate for the autologous fibrin group was faster compared to the other two groups. However all the results were not statistically significant (p value >0.05). There were no postoperative complications noted during the follow up. Autologous fibrin is comparable to HAM as a scaffold for cultivation of conjunctiva in the treatment of conjunctival defect.

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