The use of an in-house biotin-avidin linked immunosorbent assay to detect Aspergillus antigens in sera of immunocompromised patients.

S. Abdul Samad, H. Yusoff, S Fadilah S. Abdul Wahid

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1 Citation (Scopus)

Abstract

A biotin-avidin-linked immunosorbent assay was developed to detect Aspergillus antigens in sera of immunocompromised patients. The assay was based on a double antibody sandwich ELISA using polyclonal antibodies raised against water-soluble antigens of Aspergillus fumigatus. Aspergillus antigens were positive in sera of 9 of 16 (56%) patients who were studied prospectively and in 13 of 73 (19%) patients studied retrospectively. The 9 prospectively studied patients who were antigen positive were febrile neutropenic hematological malignancy patients who exhibited a high risk of acquiring invasive aspergillosis.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalMedical Journal of Malaysia
Volume56
Issue number1
Publication statusPublished - Mar 2001

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Immunosorbents
Avidin
Immunocompromised Host
Aspergillus
Biotin
Antigens
Serum
Aspergillus fumigatus
Aspergillosis
Antibodies
Hematologic Neoplasms
Fever
Enzyme-Linked Immunosorbent Assay
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "A biotin-avidin-linked immunosorbent assay was developed to detect Aspergillus antigens in sera of immunocompromised patients. The assay was based on a double antibody sandwich ELISA using polyclonal antibodies raised against water-soluble antigens of Aspergillus fumigatus. Aspergillus antigens were positive in sera of 9 of 16 (56{\%}) patients who were studied prospectively and in 13 of 73 (19{\%}) patients studied retrospectively. The 9 prospectively studied patients who were antigen positive were febrile neutropenic hematological malignancy patients who exhibited a high risk of acquiring invasive aspergillosis.",
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