The tocotrienol-rich fraction is superior to tocopherol in promoting myogenic differentiation in the prevention of replicative senescence of myoblasts

Shy Cian Khor, Azraul Mumtazah Razak, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof, Norwahidah Abdul Karim, Suzana Makpol

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aging results in a loss of muscle mass and strength. Myoblasts play an important role in maintaining muscle mass through regenerative processes, which are impaired during aging. Vitamin E potentially ameliorates age-related phenotypes. Hence, this study aimed to determine the effects of the tocotrienol-rich fraction (TRF) and á-tocopherol (ATF) in protecting myoblasts from replicative senescence and promoting myogenic differentiation. Primary human myoblasts were cultured into young and senescent stages and were then treated with TRF or ATF for 24 h, followed by an analysis of cell proliferation, senescence biomarkers, cellular morphology and differentiation. Our data showed that replicative senescence impaired the normal regenerative processes of myoblasts, resulting in changes in cellular morphology, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation and myogenic regulatory factors (MRFs) expression. Treatment with both TRF and ATF was beneficial to senescent myoblasts in reclaiming the morphology of young cells, improved cell viability and decreased SA-β-gal expression. However, only TRF treatment increased BrdU incorporation in senescent myoblasts, as well as promoted myogenic differentiation through the modulation of MRFs at the mRNA and protein levels. MYOD1 and MYOG gene expression and myogenin protein expression were modulated in the early phases of myogenic differentiation. In conclusion, the tocotrienol- rich fraction is superior to á-tocopherol in ameliorating replicative senescence-related aberration and promoting differentiation via modulation of MRFs expression, indicating Vitamin E potential in modulating replicative senescence of myoblasts.

Original languageEnglish
Article numbere0149265
JournalPLoS One
Volume11
Issue number2
DOIs
Publication statusPublished - 1 Feb 2016

Fingerprint

Tocotrienols
tocotrienols
myoblasts
Tocopherols
Myoblasts
Cell Aging
tocopherols
Myogenic Regulatory Factors
Galactosidases
Cell proliferation
Vitamin E
galactosidases
Muscle
Aging of materials
Modulation
Myogenin
vitamin E
cell proliferation
Biomarkers
Bromodeoxyuridine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The tocotrienol-rich fraction is superior to tocopherol in promoting myogenic differentiation in the prevention of replicative senescence of myoblasts. / Khor, Shy Cian; Razak, Azraul Mumtazah; Ngah, Wan Zurinah Wan; Mohd Yusof, Yasmin Anum; Abdul Karim, Norwahidah; Makpol, Suzana.

In: PLoS One, Vol. 11, No. 2, e0149265, 01.02.2016.

Research output: Contribution to journalArticle

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