Kajian tindak balas Candida albicans terhadap tekanan oksidatif

Translated title of the contribution: The study of oxidative stress responses in Candida albicans

Wan Noorhayati Wan Ibrahim, Farah Diba Abu Bakar, Nor Muhammad Mahadi, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

Abstract

The ability of a locally isolated clinical strain of C. albicans to adapt and response towards oxidative stress were investigated in this study. Treatment of C. albicans with hydrogen peroxide (H2O2) will exert an oxidative stress to C. albicans cells and affect the growth rate of this opportunistic pathogen. Cells that were grown in Yeast Peptone Dextrose (YPD) medium with the presence of 4.0 mM H2O2 gave a doubling time of 51 min/generation compared to 44 min/generation for those grown in 0.4 mM H2O2 and without H2O2. In order to determine the resistance level of C. albicans towards oxidative stress, cells were exposed to different concentration of H2O2. Results showed that percentage of cells viability decreased with the increased concentration of H2O2. These data indicated that C. albicans could overcome oxidative stress to a certain level before they were killed. To determine whether this strain exhibited a stress induce protection, cells were treated with mild oxidative stress for one hour before exposed to a stronger oxidative stress. Data obtained shows that cells that were pre-treated with mild oxidative stress showed higher percentage of survival compared to non pre-treated cells. This strongly suggested that stress induce protection was present in this C. albicans. Finally, in order to determine whether oxidative stress can induce yeast to hyphal morphogenesis in C. albicans, cells were then exposed to 0.4 mMH2O2 at 37 °C and the number of hyphal formed were compared to hyphal formation when the cells were grown at 37 °C only. However the results showed that oxidative stress failed to induce yeast to hyphal morphogenesis in this clinically isolated C. albicans strain.

Original languageUndefined/Unknown
Pages (from-to)17-21
Number of pages5
JournalSains Malaysiana
Volume35
Issue number2
Publication statusPublished - Dec 2006

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Candida albicans
Oxidative Stress
Yeasts
Morphogenesis
Peptones
Cytoprotection
Hydrogen Peroxide
Cell Survival
Glucose
Growth

Keywords

  • Candida albicans
  • Oxidative stress

ASJC Scopus subject areas

  • General

Cite this

Kajian tindak balas Candida albicans terhadap tekanan oksidatif. / Wan Ibrahim, Wan Noorhayati; Abu Bakar, Farah Diba; Mahadi, Nor Muhammad; Abd. Murad, Abdul Munir.

In: Sains Malaysiana, Vol. 35, No. 2, 12.2006, p. 17-21.

Research output: Contribution to journalArticle

Wan Ibrahim, Wan Noorhayati ; Abu Bakar, Farah Diba ; Mahadi, Nor Muhammad ; Abd. Murad, Abdul Munir. / Kajian tindak balas Candida albicans terhadap tekanan oksidatif. In: Sains Malaysiana. 2006 ; Vol. 35, No. 2. pp. 17-21.
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