The stemness gene expression of cultured human amniotic epithelial cells in serial passages.

S. F. Simat, Chua Kien Hui, H. Abdul Rahman, A. E. Tan, Geok Chin Tan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The aim of the study is to evaluate the stemness gene expression of cultured human amniotic epithelial cells (HAECs) in serial passages. HAECs obtained from human term placentae were cultured in F12:DMEM(1:1) + 10% FBS +10ng/ml EGF in serial passages (P0, P1, P2 and P4). Quantitative RT-PCR was used to assess the gene expression analysis. The results showed that cultured HAECs expressed and downregulated the stemness genes expression for Oct-4, Sox-2, Nanog3, FGF4, Rex-1, FZD-9, BST-1 ABCG2. However, vimentin and nestin gene expression were upregulated. The results suggested that cultured HAECs may have pluripotent and multipotent properties.

Original languageEnglish
Pages (from-to)53-54
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Serial Passage
Epithelial Cells
Gene Expression
Nestin
Vimentin
Epidermal Growth Factor
Placenta
Down-Regulation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The stemness gene expression of cultured human amniotic epithelial cells in serial passages. / Simat, S. F.; Kien Hui, Chua; Abdul Rahman, H.; Tan, A. E.; Tan, Geok Chin.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 53-54.

Research output: Contribution to journalArticle

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