The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1

Zangoma Maryam Haladu, Izyanti Ibrahim, Aidil Abdul Hamid

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The manner of the onset of lipid synthesis in Aurantiochytrium sp. SW1 as well as the possible role of NAD+ dependent isocitrate dehydrogenase (NAD+: ICDH) in the initiation of lipid biosynthesis were studied. The initiation of lipid synthesis in the microalgae was not associated with the cessation of growth, but commence at the early phase of growth. Substantial amount of lipid (30 %, g/g biomass) was accumulated during the active growth phase at 48 h with growth rate decreasing from 0.11 g/L/h during active growth to 0.02 g/L/h in the limited growth phase. At that period the activity of NAD+: ICDH was still detectable although it slightly decreased to 20 nmol/min/mg in 48 h from 25 nmol/min/mg at 24 h. Analysis of ammonium sulfate fractionated of NAD+: ICDH activity showed that NAD+: ICDH was not completely dependent on adenosine monophosphate (AMP) for its activity, although the presence of AMP increased the enzyme's affinity towards its substrate (isocitrate) indicated by the low Km value of the enzyme for isocitrate. While citrate acts as inhibitor of the enzyme only at high concentration. The probable implications of these properties to the regulation of lipid are discussed.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

biosynthesis
lipids
dehydrogenases
nitrogen
adenosine monophosphate
enzymes
ammonium sulfates
citrates
synthesis
biomass
inhibitors
affinity

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Haladu, Z. M., Ibrahim, I., & Abdul Hamid, A. (2018). The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020081] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027996

The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1. / Haladu, Zangoma Maryam; Ibrahim, Izyanti; Abdul Hamid, Aidil.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020081.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Haladu, ZM, Ibrahim, I & Abdul Hamid, A 2018, The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020081, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027996
Haladu ZM, Ibrahim I, Abdul Hamid A. The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020081 https://doi.org/10.1063/1.5027996
Haladu, Zangoma Maryam ; Ibrahim, Izyanti ; Abdul Hamid, Aidil. / The significance of nitrogen limited condition in the initiation of lipid biosynthesis in Aurantiochytrium sp. SW1. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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