Abstract
Optimization of fermentation medium for the production of docosahexaenoic acid (DHA) by Aurantiochytrium sp. SW1 was carried out. In this study, levels of fructose, monosodium glutamate (MSG) and sea salt were optimized for enhanced lipid and DHA production using response surface methodology (RSM). The design contains a total of 20 runs with 6 central points replication. Cultivation was carried out in 500 mL flasks containing 100 mL nitrogen limited medium at 30°C for 96h. Sequential model sum of squares (SS) revealed that the system was adequately represented by a quadratic model (p<0.0001). ANOVA results showed that fructose and MSG as a single factor has significant positive effect on the DHA content of SW1. The estimated optimal levels of the factors were 100 g/L fructose, 8 g/L MSG and 47% sea salt. Subsequent cultivation employing the suggested values confirmed that the predicted response values were experimentally achievable and reproducible, where 8.82 g/L DHA (51.34% g/g lipid) was achieved.
| Original language | English |
|---|---|
| Title of host publication | 2017 UKM FST Postgraduate Colloquium |
| Subtitle of host publication | Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium |
| Publisher | American Institute of Physics Inc. |
| Volume | 1940 |
| ISBN (Electronic) | 9780735416321 |
| DOIs | |
| Publication status | Published - 4 Apr 2018 |
| Event | 2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia Duration: 12 Jul 2017 → 13 Jul 2017 |
Other
| Other | 2017 UKM FST Postgraduate Colloquium |
|---|---|
| Country | Malaysia |
| City | Selangor |
| Period | 12/7/17 → 13/7/17 |
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ASJC Scopus subject areas
- Physics and Astronomy(all)
Cite this
The significance of fructose and MSG in affecting lipid and docosahexaenoic acid (DHA) production of Aurantiochytrium sp. SW1. / Rahman, Shariffah Nurhidayah Syed Abdul; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil.
2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020066.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - The significance of fructose and MSG in affecting lipid and docosahexaenoic acid (DHA) production of Aurantiochytrium sp. SW1
AU - Rahman, Shariffah Nurhidayah Syed Abdul
AU - Kalil, Mohd. Sahaid
AU - Abdul Hamid, Aidil
PY - 2018/4/4
Y1 - 2018/4/4
N2 - Optimization of fermentation medium for the production of docosahexaenoic acid (DHA) by Aurantiochytrium sp. SW1 was carried out. In this study, levels of fructose, monosodium glutamate (MSG) and sea salt were optimized for enhanced lipid and DHA production using response surface methodology (RSM). The design contains a total of 20 runs with 6 central points replication. Cultivation was carried out in 500 mL flasks containing 100 mL nitrogen limited medium at 30°C for 96h. Sequential model sum of squares (SS) revealed that the system was adequately represented by a quadratic model (p<0.0001). ANOVA results showed that fructose and MSG as a single factor has significant positive effect on the DHA content of SW1. The estimated optimal levels of the factors were 100 g/L fructose, 8 g/L MSG and 47% sea salt. Subsequent cultivation employing the suggested values confirmed that the predicted response values were experimentally achievable and reproducible, where 8.82 g/L DHA (51.34% g/g lipid) was achieved.
AB - Optimization of fermentation medium for the production of docosahexaenoic acid (DHA) by Aurantiochytrium sp. SW1 was carried out. In this study, levels of fructose, monosodium glutamate (MSG) and sea salt were optimized for enhanced lipid and DHA production using response surface methodology (RSM). The design contains a total of 20 runs with 6 central points replication. Cultivation was carried out in 500 mL flasks containing 100 mL nitrogen limited medium at 30°C for 96h. Sequential model sum of squares (SS) revealed that the system was adequately represented by a quadratic model (p<0.0001). ANOVA results showed that fructose and MSG as a single factor has significant positive effect on the DHA content of SW1. The estimated optimal levels of the factors were 100 g/L fructose, 8 g/L MSG and 47% sea salt. Subsequent cultivation employing the suggested values confirmed that the predicted response values were experimentally achievable and reproducible, where 8.82 g/L DHA (51.34% g/g lipid) was achieved.
UR - http://www.scopus.com/inward/record.url?scp=85045621645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045621645&partnerID=8YFLogxK
U2 - 10.1063/1.5027981
DO - 10.1063/1.5027981
M3 - Conference contribution
AN - SCOPUS:85045621645
VL - 1940
BT - 2017 UKM FST Postgraduate Colloquium
PB - American Institute of Physics Inc.
ER -