The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay

M. J. Siti Najila, A. Noor Rain, Mohamed Kamel Abdul Ghani, S. I. Syed Zahir, S. Khozirah, S. Lokman Hakim, I. Zakiah, A. K. Azizol

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalJournal of Ethnopharmacology
Volume82
Issue number2-3
DOIs
Publication statusPublished - Oct 2002

Fingerprint

Goniothalamus
Andrographis
Antimalarials
Plasmodium falciparum
Complex Mixtures
L-Lactate Dehydrogenase
Inhibitory Concentration 50
Chloroquine
Chloroform
Parasites

Keywords

  • In vitro anti-malarial activity
  • Lactate dehydrogenase assay
  • Medicinal plants
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Pharmacology

Cite this

The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay. / Siti Najila, M. J.; Noor Rain, A.; Abdul Ghani, Mohamed Kamel; Syed Zahir, S. I.; Khozirah, S.; Lokman Hakim, S.; Zakiah, I.; Azizol, A. K.

In: Journal of Ethnopharmacology, Vol. 82, No. 2-3, 10.2002, p. 239-242.

Research output: Contribution to journalArticle

Siti Najila, M. J. ; Noor Rain, A. ; Abdul Ghani, Mohamed Kamel ; Syed Zahir, S. I. ; Khozirah, S. ; Lokman Hakim, S. ; Zakiah, I. ; Azizol, A. K. / The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay. In: Journal of Ethnopharmacology. 2002 ; Vol. 82, No. 2-3. pp. 239-242.
@article{0ac0f862edc540bfb5718a5540e3c9b1,
title = "The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay",
abstract = "Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.",
keywords = "In vitro anti-malarial activity, Lactate dehydrogenase assay, Medicinal plants, Plasmodium falciparum",
author = "{Siti Najila}, {M. J.} and {Noor Rain}, A. and {Abdul Ghani}, {Mohamed Kamel} and {Syed Zahir}, {S. I.} and S. Khozirah and {Lokman Hakim}, S. and I. Zakiah and Azizol, {A. K.}",
year = "2002",
month = "10",
doi = "10.1016/S0378-8741(02)00183-6",
language = "English",
volume = "82",
pages = "239--242",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay

AU - Siti Najila, M. J.

AU - Noor Rain, A.

AU - Abdul Ghani, Mohamed Kamel

AU - Syed Zahir, S. I.

AU - Khozirah, S.

AU - Lokman Hakim, S.

AU - Zakiah, I.

AU - Azizol, A. K.

PY - 2002/10

Y1 - 2002/10

N2 - Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.

AB - Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 23.53 μg/ml against Gombak A and 21.06 μg/ml against D10.

KW - In vitro anti-malarial activity

KW - Lactate dehydrogenase assay

KW - Medicinal plants

KW - Plasmodium falciparum

UR - http://www.scopus.com/inward/record.url?scp=0036803304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036803304&partnerID=8YFLogxK

U2 - 10.1016/S0378-8741(02)00183-6

DO - 10.1016/S0378-8741(02)00183-6

M3 - Article

C2 - 12242001

AN - SCOPUS:0036803304

VL - 82

SP - 239

EP - 242

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 2-3

ER -