The role of edible bird's nest and mechanism of averting lead acetate toxicity effect on rat uterus

Abdulla A. Albishtue, Nurhusien Yimer, Md Zuki A. Zakaria, Abd Wahid Haron, Abd Salam Babji, Adamu A. Abubakar, Falah H. Baiee, Hazem Kareem Almhanna, Bahaa H. Almhanawi

Research output: Contribution to journalArticle

Abstract

Aim: This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity. Materials and Methods: Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively, for days. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA). Results: Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant - reactive oxygen species balance. Conclusion: The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles.

Original languageEnglish
Pages (from-to)1013-1021
Number of pages9
JournalVeterinary World
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

lead acetate
uterus
Uterus
Birds
toxicity
rats
proliferating cell nuclear antigen
vascular endothelial growth factors
epidermal growth factor
Proliferating Cell Nuclear Antigen
Epidermal Growth Factor
Vascular Endothelial Growth Factor A
Superoxide Dismutase
superoxide dismutase
Body Weight
body weight
Antioxidants
antioxidants
edible bird's nests
immunohistochemistry

Keywords

  • Edible bird's nest
  • Growth factors
  • Lead acetate
  • Proliferating cell nuclear antigen
  • Superoxide dismutase
  • Uterus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Albishtue, A. A., Yimer, N., Zakaria, M. Z. A., Haron, A. W., Babji, A. S., Abubakar, A. A., ... Almhanawi, B. H. (2019). The role of edible bird's nest and mechanism of averting lead acetate toxicity effect on rat uterus. Veterinary World, 12(7), 1013-1021. https://doi.org/10.14202/vetworld.2019.1013-1021

The role of edible bird's nest and mechanism of averting lead acetate toxicity effect on rat uterus. / Albishtue, Abdulla A.; Yimer, Nurhusien; Zakaria, Md Zuki A.; Haron, Abd Wahid; Babji, Abd Salam; Abubakar, Adamu A.; Baiee, Falah H.; Almhanna, Hazem Kareem; Almhanawi, Bahaa H.

In: Veterinary World, Vol. 12, No. 7, 01.01.2019, p. 1013-1021.

Research output: Contribution to journalArticle

Albishtue, AA, Yimer, N, Zakaria, MZA, Haron, AW, Babji, AS, Abubakar, AA, Baiee, FH, Almhanna, HK & Almhanawi, BH 2019, 'The role of edible bird's nest and mechanism of averting lead acetate toxicity effect on rat uterus', Veterinary World, vol. 12, no. 7, pp. 1013-1021. https://doi.org/10.14202/vetworld.2019.1013-1021
Albishtue, Abdulla A. ; Yimer, Nurhusien ; Zakaria, Md Zuki A. ; Haron, Abd Wahid ; Babji, Abd Salam ; Abubakar, Adamu A. ; Baiee, Falah H. ; Almhanna, Hazem Kareem ; Almhanawi, Bahaa H. / The role of edible bird's nest and mechanism of averting lead acetate toxicity effect on rat uterus. In: Veterinary World. 2019 ; Vol. 12, No. 7. pp. 1013-1021.
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abstract = "Aim: This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity. Materials and Methods: Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively, for days. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA). Results: Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant - reactive oxygen species balance. Conclusion: The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles.",
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AU - Yimer, Nurhusien

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AU - Haron, Abd Wahid

AU - Babji, Abd Salam

AU - Abubakar, Adamu A.

AU - Baiee, Falah H.

AU - Almhanna, Hazem Kareem

AU - Almhanawi, Bahaa H.

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N2 - Aim: This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity. Materials and Methods: Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively, for days. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA). Results: Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant - reactive oxygen species balance. Conclusion: The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles.

AB - Aim: This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity. Materials and Methods: Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively, for days. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA). Results: Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant - reactive oxygen species balance. Conclusion: The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles.

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