The role of a novel wolbachia (Rickettsiales

Anaplasmataceae) synthetic peptide, wolfar, in regulating prostaglandin levels in the hemolymph of acheta domesticus (orthoptera: Gryllidae)

Muhamad Azmi Mohammed, Ameyra Aman-Zuki, Nurul Othman Wahida, Yohsuke Tagami, Salmah Yaakop

Research output: Contribution to journalArticle

Abstract

Prostaglandins and other eicosanoids are known as regulating agents for cellular immune responses to pathogen threats in an insect’s hemolymph. A novel synthetic peptide, WolFar, was customized from a conserved region of the Wolbachia surface protein (WSP), isolated from an economically important endoparasitoid species, Fopius arisanus. WolFar consists of nine amino acids (SYY VRL QYN) and was tested on the house cricket, Acheta domesticus. Three concentrations of peptides, 0.83 mmol/L (100%), 0.63 mmol/L (75%), and 0.42 mmol/L (50%), were injected and observed for 72 h. The regulation of prostaglandin E2 (PGE2) in the hemolymph of A. domesticus was determined using ELISA and by observation of nodules in the internal system of A. domesticus. The results showed that there were significant increases in PGE2 in response to peptide injection at 24, 48, and 72 h after treatment. Furthermore, higher concentrations of peptide were directly proportional to the level of PGE2 activity. These findings were supported by the abundance of nodules that formed in the internal system and fat body of A. domesticus, detectable at 72 h into the treatment. This indicates that WolFar is able to stimulate the immune system of insects and can be further developed as a potential biopesticide.

Original languageEnglish
Pages (from-to)422-431
Number of pages10
JournalTurkish Journal of Zoology
Volume42
Issue number4
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Anaplasmataceae
Rickettsiales
Acheta domesticus
Wolbachia
synthetic peptides
Gryllidae
Orthoptera
prostaglandins
hemolymph
peptides
Fopius arisanus
entomopathogens
biopesticides
eicosanoids
surface proteins
fat body
cell-mediated immunity
immune system
enzyme-linked immunosorbent assay
injection

Keywords

  • Acheta domesticus
  • Hemolymph
  • Peptide
  • Prostaglandin
  • WSP

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The role of a novel wolbachia (Rickettsiales : Anaplasmataceae) synthetic peptide, wolfar, in regulating prostaglandin levels in the hemolymph of acheta domesticus (orthoptera: Gryllidae). / Mohammed, Muhamad Azmi; Aman-Zuki, Ameyra; Wahida, Nurul Othman; Tagami, Yohsuke; Yaakop, Salmah.

In: Turkish Journal of Zoology, Vol. 42, No. 4, 01.01.2018, p. 422-431.

Research output: Contribution to journalArticle

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abstract = "Prostaglandins and other eicosanoids are known as regulating agents for cellular immune responses to pathogen threats in an insect’s hemolymph. A novel synthetic peptide, WolFar, was customized from a conserved region of the Wolbachia surface protein (WSP), isolated from an economically important endoparasitoid species, Fopius arisanus. WolFar consists of nine amino acids (SYY VRL QYN) and was tested on the house cricket, Acheta domesticus. Three concentrations of peptides, 0.83 mmol/L (100{\%}), 0.63 mmol/L (75{\%}), and 0.42 mmol/L (50{\%}), were injected and observed for 72 h. The regulation of prostaglandin E2 (PGE2) in the hemolymph of A. domesticus was determined using ELISA and by observation of nodules in the internal system of A. domesticus. The results showed that there were significant increases in PGE2 in response to peptide injection at 24, 48, and 72 h after treatment. Furthermore, higher concentrations of peptide were directly proportional to the level of PGE2 activity. These findings were supported by the abundance of nodules that formed in the internal system and fat body of A. domesticus, detectable at 72 h into the treatment. This indicates that WolFar is able to stimulate the immune system of insects and can be further developed as a potential biopesticide.",
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