The retinoblastoma gene (rb1) in acute myeloid leukaemia: Analysis of gene rearrangements, protein expression and comparison of disease outcome

A. Rahman A. Jamal, Rosemary E. Gale, N. Shaun B Thomas, Keith Wheatley, David C. Linch

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The occurrence of retinoblastoma gene abnormalities in a large subset of various malignancies suggests an important role for this tumour suppressor gene in carcinogenesis, but this varies considerably from one tumour type to another and results in patients with acute myeloid leukaemia (AML) have been controversial. We analysed 106 AML patients and 18 normal controls for RB1 gene rearrangements and 86 AML patients for RB protein (pRB) expression. Southern blot analysis detected no gross gene rearrangements, but several restriction enzyme polymorphisms were observed. By Western blot analysis, 20 patients (23%) had no detectable pRB protein and seven (8%) had truncated pRB bands. Discordance between the DNA and protein data suggests that there may be minor deletions and point mutations in the RB1 gene or abnormalities in the proteins regulating the expression of pRB. No significant differences in the frequency of attainment of complete remission or length of survival were observed between patients with normal and abnormal pRB.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalBritish Journal of Haematology
Volume94
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Retinoblastoma Genes
Gene Rearrangement
Acute Myeloid Leukemia
Proteins
Sequence Deletion
Southern Blotting
Tumor Suppressor Genes
Point Mutation
Neoplasms
Carcinogenesis
Western Blotting

Keywords

  • AML
  • pRB
  • RB1
  • rearrangements
  • survival

ASJC Scopus subject areas

  • Hematology

Cite this

The retinoblastoma gene (rb1) in acute myeloid leukaemia : Analysis of gene rearrangements, protein expression and comparison of disease outcome. / A. Jamal, A. Rahman; Gale, Rosemary E.; Thomas, N. Shaun B; Wheatley, Keith; Linch, David C.

In: British Journal of Haematology, Vol. 94, No. 2, 1996, p. 342-351.

Research output: Contribution to journalArticle

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