The responsiveness of isolated human hand vein endothelial cells in normal pregnancy and in pre-eclampsia

Zaleha Abdullah Mahdy, H. A. Otun, W. Dunlop, J. I. Gillespie

Research output: Contribution to journalArticle

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Abstract

1. Human hand vein endothelial cells were isolated from blood obtained by traumatic venepuncture. Cells were identified as endothelial by staining with endothelium-specific antibodies. The subject groups studied were (i) non-pregnant, (ii) pregnant (mean, 35 weeks gestation) and (iii) pre-eclamptic women (mean, 35 weeks gestation). 2. Fura-2 was used to measure agonist-induced responses in intracellular Ca2+ in single endothelial cells isolated and maintained in vitro. All of the cells examined responded to adenosine triphosphate (ATP) with a large transient increase in Ca2+ followed by a sustained plateau. 3. The responses to ATP were significantly larger in the cells from pregnant women than in those from non-pregnant and pre-eclamptic women, but no other differences were observed. The amplitudes of the responses to ATP were (means ± S.E.M.) 0.56 ± 0.04, 1.42 ± 0.24 and 0.65 ± 0.09 fura-2 ratio units for cells from non-pregnant, pregnant and pre-eclamptic subjects, respectively. 4. In cells isolated from non-pregnant subjects, the amplitude of the responses to carbachol, histamine and bradykinin were all smaller than those activated by ATP: 5.1, 13.9 and 4.4%, respectively. Not all cells responded to these agonists: 25% responded to carbachol, 70.5% responded to histamine and 12.5% responded to bradykinin. Sixty-five per cent of the cells from normotensive pregnant subjects responded to bradykinin compared with 25% in the non-pregnant and 13.9% in the pre-eclamptic subjects. 5. These data suggest that there may be differences in the responsiveness of venous endothelial cells in pregnancy and that pre-eclamptic cells behave differently.

Original languageEnglish
Pages (from-to)609-617
Number of pages9
JournalJournal of Physiology
Volume508
Issue number2
DOIs
Publication statusPublished - 15 Apr 1998
Externally publishedYes

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Pre-Eclampsia
Veins
Endothelial Cells
Hand
Pregnancy
Bradykinin
Adenosine Triphosphate
Carbachol
Histamine
Phlebotomy
Fura-2
Endothelium
Pregnant Women
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Physiology

Cite this

The responsiveness of isolated human hand vein endothelial cells in normal pregnancy and in pre-eclampsia. / Abdullah Mahdy, Zaleha; Otun, H. A.; Dunlop, W.; Gillespie, J. I.

In: Journal of Physiology, Vol. 508, No. 2, 15.04.1998, p. 609-617.

Research output: Contribution to journalArticle

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abstract = "1. Human hand vein endothelial cells were isolated from blood obtained by traumatic venepuncture. Cells were identified as endothelial by staining with endothelium-specific antibodies. The subject groups studied were (i) non-pregnant, (ii) pregnant (mean, 35 weeks gestation) and (iii) pre-eclamptic women (mean, 35 weeks gestation). 2. Fura-2 was used to measure agonist-induced responses in intracellular Ca2+ in single endothelial cells isolated and maintained in vitro. All of the cells examined responded to adenosine triphosphate (ATP) with a large transient increase in Ca2+ followed by a sustained plateau. 3. The responses to ATP were significantly larger in the cells from pregnant women than in those from non-pregnant and pre-eclamptic women, but no other differences were observed. The amplitudes of the responses to ATP were (means ± S.E.M.) 0.56 ± 0.04, 1.42 ± 0.24 and 0.65 ± 0.09 fura-2 ratio units for cells from non-pregnant, pregnant and pre-eclamptic subjects, respectively. 4. In cells isolated from non-pregnant subjects, the amplitude of the responses to carbachol, histamine and bradykinin were all smaller than those activated by ATP: 5.1, 13.9 and 4.4{\%}, respectively. Not all cells responded to these agonists: 25{\%} responded to carbachol, 70.5{\%} responded to histamine and 12.5{\%} responded to bradykinin. Sixty-five per cent of the cells from normotensive pregnant subjects responded to bradykinin compared with 25{\%} in the non-pregnant and 13.9{\%} in the pre-eclamptic subjects. 5. These data suggest that there may be differences in the responsiveness of venous endothelial cells in pregnancy and that pre-eclamptic cells behave differently.",
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