The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells

Javier Munoz, Low Teck Yew, Yee J. Kok, Angela Chin, Christian K. Frese, Vanessa Ding, Andre Choo, Albert J.R. Heck

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature.

Original languageEnglish
Article number550
JournalMolecular Systems Biology
Volume7
DOIs
Publication statusPublished - 30 Nov 2011
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
Stem Cells
embryonic stem cells
Proteome
Embryonic Stem Cells
proteome
Stem cells
Proteins
cell lines
Cell Line
proteins
Protein
Antigen Presentation
Therapeutic Uses
transcriptomics
cell adhesion
Cell Adhesion
fibroblasts
Mass Spectrometry
Cells

Keywords

  • human embryonic stem cells
  • human-induced pluripotent stem cells
  • proteomics
  • quantitation

ASJC Scopus subject areas

  • Medicine(all)
  • Information Systems
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Computational Theory and Mathematics
  • Applied Mathematics

Cite this

The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. / Munoz, Javier; Teck Yew, Low; Kok, Yee J.; Chin, Angela; Frese, Christian K.; Ding, Vanessa; Choo, Andre; Heck, Albert J.R.

In: Molecular Systems Biology, Vol. 7, 550, 30.11.2011.

Research output: Contribution to journalArticle

Munoz, Javier ; Teck Yew, Low ; Kok, Yee J. ; Chin, Angela ; Frese, Christian K. ; Ding, Vanessa ; Choo, Andre ; Heck, Albert J.R. / The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. In: Molecular Systems Biology. 2011 ; Vol. 7.
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