The predictive value of the zona-free hamster egg penetration test in relation to in-vitro fertilization at various insemination concentrations

Zainul Rashid Mohd. Razi, S. B. Fishel, S. Thornton, J. A. Hall, G. Ndukwe, M. Aloum, S. D. Fleming

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The aim of the study was to evaluate the predictive value of the zona-free hamster egg penetration test (ZHEPT) for success in in-vitro fertilization (IVF) at various insemination concentrations ranging between 0.1 and > 0.6 x 106/ml. The ZHEPT was assessed using sperm samples from 87 couples undergoing IVF treatment. A similar test was simultaneously performed on the same semen sample following ionophore induction of the acrosome reaction (ZHEPTii test). Both the tests were poorly correlated with the fertilization rate of IVF at all the insemination concentrations except at > 0.6 x 106/ml, when there was good correlation between the ZHEPTii test and the fertilization rate. Following exclusion of two cases with an oocyte problem, further statistical analysis revealed that both the ZHEPT and ZHEPTii tests were poorly correlated with fertilization rate in IVF in this treatment group. This study suggests that the ZHEPT (with and without ionophore induction of the acrosome reaction) has a poor predictive value for the success of fertilization in IVF treatment at any insemination concentration.

Original languageEnglish
Pages (from-to)624-629
Number of pages6
JournalHuman Reproduction
Volume13
Issue number3
DOIs
Publication statusPublished - 1998

Fingerprint

Insemination
Herpes Zoster
Fertilization in Vitro
Cricetinae
Ovum
Fertilization
Acrosome Reaction
Ionophores
Semen
Oocytes
Spermatozoa
Therapeutics

Keywords

  • Acrosome reaction
  • Hamster egg penetration test
  • Ionophore induction
  • IVF

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

The predictive value of the zona-free hamster egg penetration test in relation to in-vitro fertilization at various insemination concentrations. / Mohd. Razi, Zainul Rashid; Fishel, S. B.; Thornton, S.; Hall, J. A.; Ndukwe, G.; Aloum, M.; Fleming, S. D.

In: Human Reproduction, Vol. 13, No. 3, 1998, p. 624-629.

Research output: Contribution to journalArticle

Mohd. Razi, Zainul Rashid ; Fishel, S. B. ; Thornton, S. ; Hall, J. A. ; Ndukwe, G. ; Aloum, M. ; Fleming, S. D. / The predictive value of the zona-free hamster egg penetration test in relation to in-vitro fertilization at various insemination concentrations. In: Human Reproduction. 1998 ; Vol. 13, No. 3. pp. 624-629.
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