The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells

Rabiatul Adawiyah Razali, Nik Ahmad Hafiz Nik Ahmad Eid, Turkambigai Jayaraman, Muhammad Asyrafi Amir Hassan, Nabilah Qistina Azlan, Nur Farhana Ismail, Nur Qisya Afifah Veronica Sainik, Muhammad Dain Yazid, Lokanathan Yogeswaran, Aminuddin Bin Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

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Abstract

Background: One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs). Methods: Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3. Results: There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05). Conclusion: The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.

Original languageEnglish
Article number197
JournalBMC Complementary and Alternative Medicine
Volume18
Issue number1
DOIs
Publication statusPublished - 26 Jun 2018

Fingerprint

Epithelial-Mesenchymal Transition
Olea
Nose
Epithelial Cells
Vimentin
Cadherins
Growth
Proteins
Transforming Growth Factor beta
Anti-Inflammatory Agents
Therapeutics

Keywords

  • Circularity
  • E-cadherin
  • EMT
  • Olive
  • TGFβ1
  • Vimentin

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells. / Razali, Rabiatul Adawiyah; Nik Ahmad Eid, Nik Ahmad Hafiz; Jayaraman, Turkambigai; Amir Hassan, Muhammad Asyrafi; Azlan, Nabilah Qistina; Ismail, Nur Farhana; Sainik, Nur Qisya Afifah Veronica; Yazid, Muhammad Dain; Yogeswaran, Lokanathan; Saim, Aminuddin Bin; Idrus, Ruszymah.

In: BMC Complementary and Alternative Medicine, Vol. 18, No. 1, 197, 26.06.2018.

Research output: Contribution to journalArticle

Razali, Rabiatul Adawiyah ; Nik Ahmad Eid, Nik Ahmad Hafiz ; Jayaraman, Turkambigai ; Amir Hassan, Muhammad Asyrafi ; Azlan, Nabilah Qistina ; Ismail, Nur Farhana ; Sainik, Nur Qisya Afifah Veronica ; Yazid, Muhammad Dain ; Yogeswaran, Lokanathan ; Saim, Aminuddin Bin ; Idrus, Ruszymah. / The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells. In: BMC Complementary and Alternative Medicine. 2018 ; Vol. 18, No. 1.
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abstract = "Background: One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs). Methods: Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05{\%} OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05{\%} OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3. Results: There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05). Conclusion: The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.",
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AU - Nik Ahmad Eid, Nik Ahmad Hafiz

AU - Jayaraman, Turkambigai

AU - Amir Hassan, Muhammad Asyrafi

AU - Azlan, Nabilah Qistina

AU - Ismail, Nur Farhana

AU - Sainik, Nur Qisya Afifah Veronica

AU - Yazid, Muhammad Dain

AU - Yogeswaran, Lokanathan

AU - Saim, Aminuddin Bin

AU - Idrus, Ruszymah

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