The Potential of Gelam Honey in Promoting the Proliferative Phase of Corneal Reepithelialization

Muhammad Fairuz Azmi, Norzana Abd. Ghafar, Jemaima Che Hamzah, Ng Sook Luan, Chua Kien Hui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: The aim of this study is to investigate the potential bene ts of Gelam honey (GH) in promoting proliferation of ex vivo cor- neal epithelial cells (CECs) and its effects on the phenotypical features.

MATERIALS AND METHODS: Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytom- etry, respectively.

RESULTS: Corneal epithelial cells cultured in 0.0015% GH-supplemented media (BM + 0.0015% GH; CM + 0.0015% GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs.

CONCLUSION: Culture media treated with 0.0015% GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential devel- opment of GH-based topical treatment for super cial corneal injury.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalWounds : a compendium of clinical research and practice
Volume29
Issue number11
Publication statusPublished - 1 Nov 2017

Fingerprint

Honey
Epithelial Cells
Keratin-3
Cornea
Cell Cycle
Cell Proliferation
Gene Expression
Phase-Contrast Microscopy
Culture Media
Real-Time Polymerase Chain Reaction
Proteins
Immunohistochemistry
Rabbits

ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

Cite this

@article{742e1fded80c47cba5ac0bec1020615a,
title = "The Potential of Gelam Honey in Promoting the Proliferative Phase of Corneal Reepithelialization",
abstract = "OBJECTIVE: The aim of this study is to investigate the potential bene ts of Gelam honey (GH) in promoting proliferation of ex vivo cor- neal epithelial cells (CECs) and its effects on the phenotypical features.MATERIALS AND METHODS: Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytom- etry, respectively.RESULTS: Corneal epithelial cells cultured in 0.0015{\%} GH-supplemented media (BM + 0.0015{\%} GH; CM + 0.0015{\%} GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs.CONCLUSION: Culture media treated with 0.0015{\%} GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential devel- opment of GH-based topical treatment for super cial corneal injury.",
author = "Azmi, {Muhammad Fairuz} and {Abd. Ghafar}, Norzana and {Che Hamzah}, Jemaima and Luan, {Ng Sook} and {Kien Hui}, Chua",
year = "2017",
month = "11",
day = "1",
language = "English",
volume = "29",
pages = "327--332",
journal = "Wounds",
issn = "1044-7946",
publisher = "HMP Communications",
number = "11",

}

TY - JOUR

T1 - The Potential of Gelam Honey in Promoting the Proliferative Phase of Corneal Reepithelialization

AU - Azmi, Muhammad Fairuz

AU - Abd. Ghafar, Norzana

AU - Che Hamzah, Jemaima

AU - Luan, Ng Sook

AU - Kien Hui, Chua

PY - 2017/11/1

Y1 - 2017/11/1

N2 - OBJECTIVE: The aim of this study is to investigate the potential bene ts of Gelam honey (GH) in promoting proliferation of ex vivo cor- neal epithelial cells (CECs) and its effects on the phenotypical features.MATERIALS AND METHODS: Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytom- etry, respectively.RESULTS: Corneal epithelial cells cultured in 0.0015% GH-supplemented media (BM + 0.0015% GH; CM + 0.0015% GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs.CONCLUSION: Culture media treated with 0.0015% GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential devel- opment of GH-based topical treatment for super cial corneal injury.

AB - OBJECTIVE: The aim of this study is to investigate the potential bene ts of Gelam honey (GH) in promoting proliferation of ex vivo cor- neal epithelial cells (CECs) and its effects on the phenotypical features.MATERIALS AND METHODS: Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytom- etry, respectively.RESULTS: Corneal epithelial cells cultured in 0.0015% GH-supplemented media (BM + 0.0015% GH; CM + 0.0015% GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs.CONCLUSION: Culture media treated with 0.0015% GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential devel- opment of GH-based topical treatment for super cial corneal injury.

UR - http://www.scopus.com/inward/record.url?scp=85050614946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050614946&partnerID=8YFLogxK

M3 - Article

C2 - 28678731

AN - SCOPUS:85050614946

VL - 29

SP - 327

EP - 332

JO - Wounds

JF - Wounds

SN - 1044-7946

IS - 11

ER -