The Pitfall of Utilizing a Commercial Biochemical Yeast Identification Kit to Detect. Candida auris

Chuan Hun Ding, Siti Fazilah Situ, Anderson Steven, Mohd Fuat Abd Razak

Research output: Contribution to journalArticle

Abstract

Candida auris is an emerging pathogenic yeast responsible for nosocomial infections with high mortality, on a global scale. A 65-year-old woman with hypovolemic shock and severe metabolic acidosis was intubated and admitted to the intensive care unit (ICU). Shortly after admission, she developed ventilator-associated pneumonia caused by multidrug-resistant Acinetobacter baumannii, which necessitated treatment with high-dose ampicillin-sulbactam. Two weeks later, a yeast was cultured from her blood. It formed pale pink colonies on CHROMagar Candida medium and produced predominantly oval budding yeast cells with the occasional rudimentary pseudohyphae on cornmeal agar. ID 32 C identified the yeast as Candida sake However, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and sequencing of the D1/D2 region of the 28S rRNA gene identified the yeast as C. auris.

Original languageEnglish
Pages (from-to)546-549
Number of pages4
JournalAnnals of clinical and laboratory science
Volume49
Issue number4
Publication statusPublished - 1 Sep 2019

Fingerprint

Candida
Yeast
Yeasts
Ventilator-Associated Pneumonia
Acinetobacter baumannii
Saccharomycetales
Cross Infection
Acidosis
Intensive care units
rRNA Genes
Agar
Intensive Care Units
Shock
Mass Spectrometry
Lasers
Ionization
Mass spectrometry
Desorption
Blood
Genes

Keywords

  • 28S rRNA
  • Candia sake
  • Candida auris
  • CHROMagar
  • ID 32 C
  • MALDI-TOF MS

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology
  • Molecular Biology
  • Hematology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

The Pitfall of Utilizing a Commercial Biochemical Yeast Identification Kit to Detect. Candida auris. / Ding, Chuan Hun; Situ, Siti Fazilah; Steven, Anderson; Razak, Mohd Fuat Abd.

In: Annals of clinical and laboratory science, Vol. 49, No. 4, 01.09.2019, p. 546-549.

Research output: Contribution to journalArticle

Ding, Chuan Hun ; Situ, Siti Fazilah ; Steven, Anderson ; Razak, Mohd Fuat Abd. / The Pitfall of Utilizing a Commercial Biochemical Yeast Identification Kit to Detect. Candida auris. In: Annals of clinical and laboratory science. 2019 ; Vol. 49, No. 4. pp. 546-549.
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