The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts

Yew Keong Choong, Mohamed Mustapha Noordin, Suhaila Mohamed, Abdul Manaf Ali, Nor Aini B Umar, Chow Chin Tong

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL-1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL-1.

    Original languageEnglish
    Pages (from-to)115-125
    Number of pages11
    JournalInternational Journal of Medicinal Mushrooms
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Ganoderma
    Complex Mixtures
    Ganoderma lucidum
    karsts
    breast neoplasms
    apoptosis
    Reishi
    Apoptosis
    Breast Neoplasms
    extracts
    Inhibitory Concentration 50
    inhibitory concentration 50
    Tsuga
    Ganoderma tsugae
    acridine orange
    Acridine Orange
    Propidium
    tetrazolium
    fruiting bodies
    Transmission Electron Microscopy

    Keywords

    • Apoptosis
    • Ganoderma
    • IC
    • Ling Zhi mushrooms
    • MDA-MB-435
    • Medicinal mushrooms
    • Mycelium
    • Necrosis

    ASJC Scopus subject areas

    • Molecular Medicine
    • Plant Science
    • Pharmacology, Toxicology and Pharmaceutics(all)

    Cite this

    The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts. / Choong, Yew Keong; Noordin, Mohamed Mustapha; Mohamed, Suhaila; Ali, Abdul Manaf; Umar, Nor Aini B; Tong, Chow Chin.

    In: International Journal of Medicinal Mushrooms, Vol. 10, No. 2, 2008, p. 115-125.

    Research output: Contribution to journalArticle

    Choong, Yew Keong ; Noordin, Mohamed Mustapha ; Mohamed, Suhaila ; Ali, Abdul Manaf ; Umar, Nor Aini B ; Tong, Chow Chin. / The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts. In: International Journal of Medicinal Mushrooms. 2008 ; Vol. 10, No. 2. pp. 115-125.
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    abstract = "The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL-1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL-1.",
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    AU - Tong, Chow Chin

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