The isolation and characterization of an endochitinase gene from a Malaysian isolate of Trichoderma sp

Kalaivani K. Nadarajah, Hamdia Z. Ali, Nurfarahana Syuhada Omar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chitinases have been reported to be capable of hydrolyzing chitin by splitting their ß-1,4-glucosidic bonds. The chitinases are divided into the exo and endochitinases. The aim of this study was to isolate and characterize an endochitinase gene from a local isolate of Trichoderma spp. that was isolated from the Malaysian soil samples. In total, six highly antagonistic Trichoderma isolates were screened for chitinolytic activity via dual plate method and greenhouse studies. Trichorderma isolate T2 was identified as a target for isolation of an endochitinase gene due to its high chitinolytic enzyme activity by observing the degradation of chitin substrates. The genomic DNA of Trichoderma isolate T2 was extracted, amplified and sequenced. The putative endochitinase gene, ChitT2 was then subjected to in silico analysis to obtain physico-chemical, evolutionary and structural information of this protein. The ChitT2 protein sequence had 352 amino acids and showed 99% homology to Trichoderma harzianum endochitinase Chit36Y. The maximum parsimony analysis showed that ChitT2 protein was clustered into Group V with other fungi. Ultimately, the in silico analysis of ChitT2 implicated the involvement of this gene/protein in chitin catabolism.

Original languageEnglish
Pages (from-to)711-721
Number of pages11
JournalAustralian Journal of Crop Science
Volume8
Issue number5
Publication statusPublished - 2014

Fingerprint

Trichoderma
chitin
chitinase
genes
proteins
Trichoderma harzianum
amino acid sequences
soil sampling
enzyme activity
greenhouses
genomics
amino acids
fungi
metabolism
degradation
DNA
methodology

Keywords

  • Conserved domains
  • Endochitinase
  • Glycosyl hydrolases
  • Trichorderma

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

The isolation and characterization of an endochitinase gene from a Malaysian isolate of Trichoderma sp. / K. Nadarajah, Kalaivani; Ali, Hamdia Z.; Omar, Nurfarahana Syuhada.

In: Australian Journal of Crop Science, Vol. 8, No. 5, 2014, p. 711-721.

Research output: Contribution to journalArticle

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