Interaksi antara lusiferin kunang-kunang bersama dna G-kuadrupleks dan CT-DNA

Translated title of the contribution: The interaction between firefly luciferin with G-quadruplex DNA and CT-DNA

Nurul Huda Abd Karim, Roshatiara Shamsuddin, Aida Mastura Mohd Yussof, Nur Amirah Harunar Rashid

Research output: Contribution to journalArticle

Abstract

Firefly bioluminescence gives fascinating challenges and great interest for fundamental sciences. The luciferase enzyme which emits colour for firefly bioluminiscence has been intensively studied by chemists in various applications. Luciferin the substrate of luciferase, is also responsible for the characteristic of yellow light emission from many firefly species. In recent years, the binding of small molecules with DNA has been receiving great attention due to the potential of such binding to inhibit cancer cell growth. G-quadruplex DNA has been discovered as an interesting target since stabilizing its formation in telomere can inhibit the elongation of telomere in cancer cell where enzyme telomerase is 85% activated. Here, we investigate the binding properties between luciferin compound with G-quadruplex DNA. The binding interaction was studied using UV/Vis spectra and fluorescence. The results indicate that luciferin is more selective to stabilize G-quadruplex DNA with Kb = 2.02 ± 0.59 × 105 M-1 compared to CT-DNA.

Original languageMalay
Pages (from-to)654-659
Number of pages6
JournalMalaysian Journal of Analytical Sciences
Volume23
Issue number4
DOIs
Publication statusPublished - 1 Aug 2019

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Firefly Luciferin
DNA
Luciferases
Bioluminescence
Telomerase
Light emission
Cell growth
Enzymes
Elongation
Fluorescence
Cells
Color
Molecules
Substrates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Interaksi antara lusiferin kunang-kunang bersama dna G-kuadrupleks dan CT-DNA. / Abd Karim, Nurul Huda; Shamsuddin, Roshatiara; Yussof, Aida Mastura Mohd; Rashid, Nur Amirah Harunar.

In: Malaysian Journal of Analytical Sciences, Vol. 23, No. 4, 01.08.2019, p. 654-659.

Research output: Contribution to journalArticle

Abd Karim, Nurul Huda ; Shamsuddin, Roshatiara ; Yussof, Aida Mastura Mohd ; Rashid, Nur Amirah Harunar. / Interaksi antara lusiferin kunang-kunang bersama dna G-kuadrupleks dan CT-DNA. In: Malaysian Journal of Analytical Sciences. 2019 ; Vol. 23, No. 4. pp. 654-659.
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AB - Firefly bioluminescence gives fascinating challenges and great interest for fundamental sciences. The luciferase enzyme which emits colour for firefly bioluminiscence has been intensively studied by chemists in various applications. Luciferin the substrate of luciferase, is also responsible for the characteristic of yellow light emission from many firefly species. In recent years, the binding of small molecules with DNA has been receiving great attention due to the potential of such binding to inhibit cancer cell growth. G-quadruplex DNA has been discovered as an interesting target since stabilizing its formation in telomere can inhibit the elongation of telomere in cancer cell where enzyme telomerase is 85% activated. Here, we investigate the binding properties between luciferin compound with G-quadruplex DNA. The binding interaction was studied using UV/Vis spectra and fluorescence. The results indicate that luciferin is more selective to stabilize G-quadruplex DNA with Kb = 2.02 ± 0.59 × 105 M-1 compared to CT-DNA.

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