The importance of GDN motif in domain III and GXGXG motif in domain VI of L protein in replication of Nipah virus

Siti Aishah Jalani, Nazlina Ibrahim

Research output: Contribution to journalArticle

Abstract

This study aims to determine the importance of conserved GDN motif in domain III and GXGXG motif in domain VI in Nipah virus (NiV) L protein. Four mutated L genes produced in an earlier study were inserted individually into plasmid pCITE. Optimised transfection protocol was successful in transfecting these plasmids, two helper plasmids (coding for N and P protein), NiV minigenome containing chloramphenicol acetyltransferase (CAT) reporter gene and T7 promoter. Successful in vitro transcription/translation in the NiV minireplicon system was monitored by CAT expression. In conclusion, GXGXG motif was important in the NiV minireplicon system but change of GDN motif does not affect L protein.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

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Nipah Virus
Chloramphenicol O-Acetyltransferase
Plasmids
Proteins
Reporter Genes
Transfection
Genes

Keywords

  • Conserved domain
  • GDN motif of domain III
  • GXGXG motif of domain VI
  • L gene Nipah virus minireplication system

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

The importance of GDN motif in domain III and GXGXG motif in domain VI of L protein in replication of Nipah virus. / Jalani, Siti Aishah; Ibrahim, Nazlina.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 585-589.

Research output: Contribution to journalArticle

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