The host pathogen interactions observed between Fusarium sp. F2 and rice

Kalaivani K. Nadarajah, Hamdia Z. Ali, Nurulhidayah Mat Muni

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An ITS verification was conducted on a fungal isolate (Fusarium sp F2) obtained from diseased culms of MR219. The ITS sequence analysis determined the isolate be a member of the Fusarium incarnatum-equisetti complex (FIEC). This isolate was then used to study cellular interactions in three rice varieties (Pongsu Seribu, Maswangi and MR219) where the microscopic observation of DAB stained infected tissues displayed inter and intracellular proliferation in all varieties with low to high levels of ROIs detected in and around infected tissues. Further to the cellular observation, we studied the variation in expression levels of a defense related gene, PR-1b where the level of ROIs expressed within the infected tissue corresponds directly to the levels of gene expressed. Pongsu Seribu was the most resistant rice variety to Fusarium sp F2 infections based on disease severity, ROI levels, gene expression profiles and fold increase.

Original languageEnglish
Pages (from-to)572-580
Number of pages9
JournalPlant OMICS
Volume8
Issue number6
Publication statusPublished - 2015

Fingerprint

host-pathogen relationships
Fusarium
rice
Fusarium incarnatum
disease severity
genes
sequence analysis
gene expression
infection
tissues

Keywords

  • Fusarium sp
  • ITS analysis
  • Pathogenesis Related (PR) protein
  • Reactive oxygen intermediates (ROI)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

The host pathogen interactions observed between Fusarium sp. F2 and rice. / K. Nadarajah, Kalaivani; Ali, Hamdia Z.; Muni, Nurulhidayah Mat.

In: Plant OMICS, Vol. 8, No. 6, 2015, p. 572-580.

Research output: Contribution to journalArticle

K. Nadarajah, Kalaivani ; Ali, Hamdia Z. ; Muni, Nurulhidayah Mat. / The host pathogen interactions observed between Fusarium sp. F2 and rice. In: Plant OMICS. 2015 ; Vol. 8, No. 6. pp. 572-580.
@article{f91cdc2854aa4c85a7ccf549de6435c1,
title = "The host pathogen interactions observed between Fusarium sp. F2 and rice",
abstract = "An ITS verification was conducted on a fungal isolate (Fusarium sp F2) obtained from diseased culms of MR219. The ITS sequence analysis determined the isolate be a member of the Fusarium incarnatum-equisetti complex (FIEC). This isolate was then used to study cellular interactions in three rice varieties (Pongsu Seribu, Maswangi and MR219) where the microscopic observation of DAB stained infected tissues displayed inter and intracellular proliferation in all varieties with low to high levels of ROIs detected in and around infected tissues. Further to the cellular observation, we studied the variation in expression levels of a defense related gene, PR-1b where the level of ROIs expressed within the infected tissue corresponds directly to the levels of gene expressed. Pongsu Seribu was the most resistant rice variety to Fusarium sp F2 infections based on disease severity, ROI levels, gene expression profiles and fold increase.",
keywords = "Fusarium sp, ITS analysis, Pathogenesis Related (PR) protein, Reactive oxygen intermediates (ROI)",
author = "{K. Nadarajah}, Kalaivani and Ali, {Hamdia Z.} and Muni, {Nurulhidayah Mat}",
year = "2015",
language = "English",
volume = "8",
pages = "572--580",
journal = "Plant OMICS",
issn = "1836-0661",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "6",

}

TY - JOUR

T1 - The host pathogen interactions observed between Fusarium sp. F2 and rice

AU - K. Nadarajah, Kalaivani

AU - Ali, Hamdia Z.

AU - Muni, Nurulhidayah Mat

PY - 2015

Y1 - 2015

N2 - An ITS verification was conducted on a fungal isolate (Fusarium sp F2) obtained from diseased culms of MR219. The ITS sequence analysis determined the isolate be a member of the Fusarium incarnatum-equisetti complex (FIEC). This isolate was then used to study cellular interactions in three rice varieties (Pongsu Seribu, Maswangi and MR219) where the microscopic observation of DAB stained infected tissues displayed inter and intracellular proliferation in all varieties with low to high levels of ROIs detected in and around infected tissues. Further to the cellular observation, we studied the variation in expression levels of a defense related gene, PR-1b where the level of ROIs expressed within the infected tissue corresponds directly to the levels of gene expressed. Pongsu Seribu was the most resistant rice variety to Fusarium sp F2 infections based on disease severity, ROI levels, gene expression profiles and fold increase.

AB - An ITS verification was conducted on a fungal isolate (Fusarium sp F2) obtained from diseased culms of MR219. The ITS sequence analysis determined the isolate be a member of the Fusarium incarnatum-equisetti complex (FIEC). This isolate was then used to study cellular interactions in three rice varieties (Pongsu Seribu, Maswangi and MR219) where the microscopic observation of DAB stained infected tissues displayed inter and intracellular proliferation in all varieties with low to high levels of ROIs detected in and around infected tissues. Further to the cellular observation, we studied the variation in expression levels of a defense related gene, PR-1b where the level of ROIs expressed within the infected tissue corresponds directly to the levels of gene expressed. Pongsu Seribu was the most resistant rice variety to Fusarium sp F2 infections based on disease severity, ROI levels, gene expression profiles and fold increase.

KW - Fusarium sp

KW - ITS analysis

KW - Pathogenesis Related (PR) protein

KW - Reactive oxygen intermediates (ROI)

UR - http://www.scopus.com/inward/record.url?scp=84951740783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951740783&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 572

EP - 580

JO - Plant OMICS

JF - Plant OMICS

SN - 1836-0661

IS - 6

ER -