The growth yield of Desulfovibrio desulfuricans M6 on different substrates

Doo Hyun Park, Chul Su Shin, Byung Hong Kim, Pyung Kyun Shin

Research output: Contribution to journalArticle

Abstract

Growth yield of Desulfovibrio desulfuricans M6 was measured using different substrates. The cell yield of fermentative growth on pyruvate was 6.22 g cell mol-1 pyruvate. Since 1 ATP is available from substrate-level phosphorylation from the oxidation of pyruvate to acetate, YATP of the bacterium should be the same as Ypyruvate (6.22 g cell mol-1 ATP). The cell yields of the bacterium on different electron donors were measured with sulfate as the electron acceptor. Cell yields on lactate, pyruvate and H2 were 9.39, 13.76 and 8.45 g cell mol-1 substrate, respectively. From these figures ATP available from electron-transport phosphorylation (ETP) of the electron donors used was calculated. ATP produced by ETP of each electron donnor were 1.71 from pyruvate, 1.51 from lactate and 1.76 from H2. These values show that electrons from the oxidation of lactate to pyruvate are consumed to reduce sulfate through a reverse electron transport mechanism requiring 0.2 ATP for each pair of electrons. Based on these results, discussions are made on the electron transport mechanism in the bacterium.

Original languageEnglish
Pages (from-to)232-237
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume6
Issue number4
Publication statusPublished - Aug 1996
Externally publishedYes

Fingerprint

Desulfovibrio desulfuricans
Pyruvic Acid
Adenosinetriphosphate
Electrons
Electron Transport
Adenosine Triphosphate
Phosphorylation
Substrates
Growth
Lactic Acid
Bacteria
Sulfates
Oxidation
Acetates

Keywords

  • Anaerobic respiration
  • Bioenergetics
  • Desulfovibrio desulfuricans M6
  • Molar growth yield
  • Sulfate-reduction
  • Y

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Park, D. H., Shin, C. S., Kim, B. H., & Shin, P. K. (1996). The growth yield of Desulfovibrio desulfuricans M6 on different substrates. Journal of Microbiology and Biotechnology, 6(4), 232-237.

The growth yield of Desulfovibrio desulfuricans M6 on different substrates. / Park, Doo Hyun; Shin, Chul Su; Kim, Byung Hong; Shin, Pyung Kyun.

In: Journal of Microbiology and Biotechnology, Vol. 6, No. 4, 08.1996, p. 232-237.

Research output: Contribution to journalArticle

Park, DH, Shin, CS, Kim, BH & Shin, PK 1996, 'The growth yield of Desulfovibrio desulfuricans M6 on different substrates', Journal of Microbiology and Biotechnology, vol. 6, no. 4, pp. 232-237.
Park, Doo Hyun ; Shin, Chul Su ; Kim, Byung Hong ; Shin, Pyung Kyun. / The growth yield of Desulfovibrio desulfuricans M6 on different substrates. In: Journal of Microbiology and Biotechnology. 1996 ; Vol. 6, No. 4. pp. 232-237.
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