The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat

Mohd Firdaus Mohd Raih, Noor Haza Fazlin Hashim, Izwan Bharudin, Mohd Faizal Abu Bakar, Kie Kyon Huang, Halimah Alias, Bernard K.B. Lee, Mohd Noor Mat Isa, Shuhaila Mat-Sharani, Suhaila Sulaiman, Lih Jinq Tay, Radziah Zolkefli, Yusuf Muhammad Noor, Douglas Sie Nguong Law, Siti Hamidah Abdul Rahman, Rosli Md-Illias, Farah Diba Abu Bakar, Nazalan Najimudin, Abdul Munir Abd. Murad, Nor Muhammad Mahadi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival.

Original languageEnglish
Article numbere0189947
JournalPLoS One
Volume13
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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Antarctica
Ecosystem
Genes
Genome
Temperature
genome
habitats
temperature
Antarctic Regions
Proteins
genes
Ice
microbiology
Microbiology
Recombinant Proteins
recombinant proteins
Sequence Analysis
ice
sequence analysis
proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. / Mohd Raih, Mohd Firdaus; Hashim, Noor Haza Fazlin; Bharudin, Izwan; Bakar, Mohd Faizal Abu; Huang, Kie Kyon; Alias, Halimah; Lee, Bernard K.B.; Isa, Mohd Noor Mat; Mat-Sharani, Shuhaila; Sulaiman, Suhaila; Tay, Lih Jinq; Zolkefli, Radziah; Noor, Yusuf Muhammad; Law, Douglas Sie Nguong; Rahman, Siti Hamidah Abdul; Md-Illias, Rosli; Abu Bakar, Farah Diba; Najimudin, Nazalan; Abd. Murad, Abdul Munir; Mahadi, Nor Muhammad.

In: PLoS One, Vol. 13, No. 1, e0189947, 01.01.2018.

Research output: Contribution to journalArticle

Mohd Raih, MF, Hashim, NHF, Bharudin, I, Bakar, MFA, Huang, KK, Alias, H, Lee, BKB, Isa, MNM, Mat-Sharani, S, Sulaiman, S, Tay, LJ, Zolkefli, R, Noor, YM, Law, DSN, Rahman, SHA, Md-Illias, R, Abu Bakar, FD, Najimudin, N, Abd. Murad, AM & Mahadi, NM 2018, 'The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat', PLoS One, vol. 13, no. 1, e0189947. https://doi.org/10.1371/journal.pone.0189947
Mohd Raih, Mohd Firdaus ; Hashim, Noor Haza Fazlin ; Bharudin, Izwan ; Bakar, Mohd Faizal Abu ; Huang, Kie Kyon ; Alias, Halimah ; Lee, Bernard K.B. ; Isa, Mohd Noor Mat ; Mat-Sharani, Shuhaila ; Sulaiman, Suhaila ; Tay, Lih Jinq ; Zolkefli, Radziah ; Noor, Yusuf Muhammad ; Law, Douglas Sie Nguong ; Rahman, Siti Hamidah Abdul ; Md-Illias, Rosli ; Abu Bakar, Farah Diba ; Najimudin, Nazalan ; Abd. Murad, Abdul Munir ; Mahadi, Nor Muhammad. / The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. In: PLoS One. 2018 ; Vol. 13, No. 1.
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