The formation of human auricular cartilage from microtic tissue

An in vivo study

Mohamad Fikeri bin Ishak, Bee See Goh, Chua Kien Hui, Asma Abdullah, Lokman bin Saim, Aminuddin bin Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: This study aimed to isolate, culture-expand and characterize the chondrocytes isolated from microtic cartilage and evaluate its potential as a cell source for ear cartilage reconstruction. Specific attention was to construct the auricular cartilage tissue by using fibrin as scaffold. Study design: Cell culture experiment with the use of microtic chondrocytes. Design: Cell culture experiment with the use of microtic chondrocytes. Methods: After ear reconstructive surgery at the Universiti Kebangsaan Malaysia Medical Center, chondrocytes were isolated from microtic cartilage. Chondrocytes isolated from the tissue were cultured expanded until passage 4 (P4). Upon confluency at P4, chondrocytes were harvested and tissue engineered constructs were made with human plasma polymerized to fibrin. Constructs formed later is implanted at the dorsal part of nude mice for 8 weeks, followed by post-implantation evaluation with histology staining (Hematoxylin and Eosin (H&E) and Safranin O), immunohistochemistry and RT-PCR for chondrogenic associated genes expression level. Results: Under gross assessment, the construct after 8 weeks of implantation showed similar physical characteristics that of cartilage. Histological staining showed abundant lacunae cells embedded in extracellular matrix similar to that of native cartilage. Safranin O staining showed positive staining which indicates the presence of proteoglycan-rich matrix. Immunohistochemistry analysis showed the strong positive staining for collagen type II, the specific collagen type in the cartilage. Gene expression quantification showed no significant differences in the expression of chondrogenic gene used which is collagen type I, collagen type II, aggrecan core protein (ACP), elastin and sox9 genes when compared to construct formed from normal auricular tissue. Conclusion: Chondrocytes isolated from microtia cartilage has the potential to be used as an alternative cell source for external ear reconstruction in future clinical application.

Original languageEnglish
Pages (from-to)1634-1639
Number of pages6
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume79
Issue number10
DOIs
Publication statusPublished - 1 Oct 2015

Fingerprint

Ear Cartilage
Chondrocytes
Cartilage
Staining and Labeling
Collagen Type II
Fibrin
Gene Expression
Reconstructive Surgical Procedures
Cell Culture Techniques
Immunohistochemistry
External Ear
Aggrecans
Elastin
Malaysia
Proteoglycans
Hematoxylin
Eosine Yellowish-(YS)
Collagen Type I
Nude Mice
Extracellular Matrix

Keywords

  • Auricular chondrocytes
  • Ear
  • Fibrin
  • Microtia
  • Tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Pediatrics, Perinatology, and Child Health

Cite this

The formation of human auricular cartilage from microtic tissue : An in vivo study. / Ishak, Mohamad Fikeri bin; Goh, Bee See; Kien Hui, Chua; Abdullah, Asma; Saim, Lokman bin; Saim, Aminuddin bin; Idrus, Ruszymah.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 79, No. 10, 01.10.2015, p. 1634-1639.

Research output: Contribution to journalArticle

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AU - Saim, Aminuddin bin

AU - Idrus, Ruszymah

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