The fluorometric response of cyanobateria to short exposure of heavy metal

Wong Ling Shing, Yook Heng Lee, Salmijah Surif

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study is focused on the response of cyanobacteria Anabaena torulosa to a short exposure to copper. Measurement was based on the differential of the fluorescence signal of the organism before and after the exposure to copper, with excitation and emission set at 526 nm and 648 nm respectively. To optimize the yield of fluorescence signal, the day-7 culture of cell suspension was used with the density of OD 700nm= 0.45 A, with short exposure time of 8 minutes to Cu. Tests on different concentration of Cu showed the cyanobacteria fluorescence yields formed a linear range from 1 -10 μg/L. The cyanobacteria could be a good candidate for biosensor for producing signals under a short exposure to low concentration of Cu.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalAdvances in Environmental Biology
Volume6
Issue number1
Publication statusPublished - Jan 2012

Fingerprint

Cyanobacteria
Heavy Metals
heavy metals
Fluorescence
heavy metal
fluorescence
cyanobacterium
Anabaena torulosa
Copper
copper
Anabaena
biosensors
Biosensing Techniques
cell suspension culture
exposure duration
Suspensions
Cell Culture Techniques
organisms
exposure
testing

Keywords

  • Cyanobacteria
  • Fluorometric response
  • Heavy metal
  • Short exposure time

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

The fluorometric response of cyanobateria to short exposure of heavy metal. / Shing, Wong Ling; Lee, Yook Heng; Surif, Salmijah.

In: Advances in Environmental Biology, Vol. 6, No. 1, 01.2012, p. 103-108.

Research output: Contribution to journalArticle

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